Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   SEED AND PLANT   2009 , Volume 24 , Number 4; Page(s) 739 To 748.
 
Paper: 

PROLIFERATION OF EMBRYOGENIC CALLUS, INDUCTION OF SOMATIC EMBRYO AND PLANT REGENERATION IN CARNATION (DIANTHUS CARYOPHYLLUS L.)

 
 
Author(s):  KARAMI O., KARIMI KORDESTANI G., MOHAMMADI MOHAMMAD
 
* 
 
Abstract: 

Proliferation of embryogenic calli and production of healthy plants are the most powerful aspects of somatic embryogenesis. The aim of study was proliferation of embryogenic calli followed by regeneration and production of healthy plants in two carnation cultivars Nelson and Spirit. Proliferation of embryogenic calli was achieved in two cultivars of carnation. MS culture medium used for embryogenic callus formation was supplemented with 30 gl-1 sucrose, 2 mgl-1 2, 4-D and 0.2 mgl-1 BA. Proliferation of embryogenic calli was observed on MS basal medium containing different concentrations (0.2, 0.5, 1, 2, 4, 6 mg1-1) of picloram. Maximum proliferation of calli was obtained in concentration of 0.5 mg1-1 picloram. Increasing the number of calli subcultutes, increased the rate of embryogenesis. Proliferated calli were transfered to hormone free MS medium containing 15, 30, 60, 90 and 120 g1-1 mannitol. Maximum embryogenesis was obtained using 90 g1-1 mannitol. Cotyledonary somatic embryos were evoluted into plantlets when transferred in the half-strength MS medium without growth regulators. Plantlets were also continued to grow under greenhouse condition.

 
Keyword(s): CARNATION, PROLIFERATION, CALLUS, SOMATIC EMBRYO, PICLORAM, MANNITOL
 
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
  • Not Registered.
 
+ Click to Cite.
APA: Copy

KARAMI, O., & KARIMI KORDESTANI, G., & MOHAMMADI, M. (2009). PROLIFERATION OF EMBRYOGENIC CALLUS, INDUCTION OF SOMATIC EMBRYO AND PLANT REGENERATION IN CARNATION (DIANTHUS CARYOPHYLLUS L.). SEED AND PLANT, 24(4), 739-748. https://www.sid.ir/en/journal/ViewPaper.aspx?id=146378



Vancouver: Copy

KARAMI O., KARIMI KORDESTANI G., MOHAMMADI MOHAMMAD. PROLIFERATION OF EMBRYOGENIC CALLUS, INDUCTION OF SOMATIC EMBRYO AND PLANT REGENERATION IN CARNATION (DIANTHUS CARYOPHYLLUS L.). SEED AND PLANT. 2009 [cited 2021November30];24(4):739-748. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=146378



IEEE: Copy

KARAMI, O., KARIMI KORDESTANI, G., MOHAMMADI, M., 2009. PROLIFERATION OF EMBRYOGENIC CALLUS, INDUCTION OF SOMATIC EMBRYO AND PLANT REGENERATION IN CARNATION (DIANTHUS CARYOPHYLLUS L.). SEED AND PLANT, [online] 24(4), pp.739-748. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=146378.



 
 
Persian Abstract Yearly Visit 42
 
 
Latest on Blog
Enter SID Blog