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Paper Information

Journal:   INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST)   SUMMER 2009 , Volume 6 , Number 3 (23); Page(s) 435 To 442.
 
Paper: 

ISOLATION AND CHARACTERIZATION OF A NOVEL NATIVE BACILLUS STRAIN CAPABLE OF DEGRADING DIESEL FUEL

 
 
Author(s):  YOUSEFI KEBRIA D., KHODADADI A.*, GANJIDOUST H., BADKOUBI A., AMOUZGAR M.A.
 
* DEPARTMENT OF CIVIL ENGINEERING, FACULTY OF ENVIRONMENTAL ENGINEERING, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

The ability of native bacteria to utilize diesel fuel as the sole carbon and energy source was investigated in this research. Ten bacterial strains were isolated from the oil refinery field in Tehran, Iran. Two biodegradation experiments were performed in low and high (500 and 10000ppm, respectively) concentration of diesel fuel for 15 days. Only two isolates were able to efficiently degrade the petroleum hydrocarbons in the first test and degraded 86.67% and, 80.60% of diesel fuel, respectively. The secondary experiment was performed to investigate the toxicity effect of diesel fuel at high concentration (10000ppm). Only one strain was capable to degrade 85.20 % of diesel fuel at the same time (15 days). Phenotype and phylogeny analysis of this strain was characterized and identified as diesel degrading bacteria, based on gram staining, biochemical tests, 16S rRNA gene sequence analysis. These results indicate that this new strain was Bacillus sp. and could be considered as Bacillus Cereus with 98 % 16 S rRNA gene sequence similarity. The results indicate that native strains have great potential for in situ remediation of diesel-contaminated soils in oil refinery sites.

 
Keyword(s): BACILLUS CEREUS, BACTERIA, BIODEGRADATION, BIOREMEDIATION, PETROLEUM
 
 
References: 
 
Citations: 
 
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APA: Copy

YOUSEFI KEBRIA, D., & KHODADADI, A., & GANJIDOUST, H., & BADKOUBI, A., & AMOUZGAR, M. (2009). ISOLATION AND CHARACTERIZATION OF A NOVEL NATIVE BACILLUS STRAIN CAPABLE OF DEGRADING DIESEL FUEL. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST), 6(3 (23)), 435-442. https://www.sid.ir/en/journal/ViewPaper.aspx?id=141074



Vancouver: Copy

YOUSEFI KEBRIA D., KHODADADI A., GANJIDOUST H., BADKOUBI A., AMOUZGAR M.A.. ISOLATION AND CHARACTERIZATION OF A NOVEL NATIVE BACILLUS STRAIN CAPABLE OF DEGRADING DIESEL FUEL. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST). 2009 [cited 2021August05];6(3 (23)):435-442. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=141074



IEEE: Copy

YOUSEFI KEBRIA, D., KHODADADI, A., GANJIDOUST, H., BADKOUBI, A., AMOUZGAR, M., 2009. ISOLATION AND CHARACTERIZATION OF A NOVEL NATIVE BACILLUS STRAIN CAPABLE OF DEGRADING DIESEL FUEL. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST), [online] 6(3 (23)), pp.435-442. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=141074.



 
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