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Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   December 2001 , Volume 37 , Number 3-4; Page(s) 197 To 208.
 
Paper: 

PHENOTYPIC DIVERSITY IN FUSARIUM GRAMINEARUM ISOLATES AND INTRODUCTION OF A NEW GENETIC MARKER FOR THE SPECIES

 
 
Author(s):  SAFAEE N., ALI ZADEH A.
 
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Abstract: 

Phenotypic diversity of 54 isolates of Fusarium graminearum, the causal agent of wheat head blight, was studied using pectinase, cellulase, lipase, fatty acid esterase, protease (casein hydrolysis) and gelatinase activities. On the basis of these enzymatic activities, the isolates were differentiated into four categories. Also a new genetic marker for this species was developed using selenate mutagenesis. A mycelial disk of each isolate was transferred onto a minimal medium containing I g sodium selenate and 0.1 g taurine. Cultures were monitored daily for two weeks starting one week after incubation and the selenate resistant mutants were selected. Two classes of mutants A and B, were identified. Isolates of class A had a high germination rate ranging from 61-91% (average 67%), produced fast growing colonies on MM+SeO4 and were resistant to chromate. Class B mutants had a low germination rate <35%), produced restricted slow growing colonies (4-2mm) however, like calss A isolates were resistant to chromate. No mutant belonging to class C and susceptible to chromate was recovered. This is the first report on production of selenate resistant mutants in F. graminearum. Such mutants are potentially useful for genetic analysis in this species.

 
Keyword(s): PHENOTYPIC DIVERSITY, FUSARIUM GRAMINEARUM, FUSARIUM BEAD BLIGHT, GENETIC MARKER
 
References: 
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