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Paper Information

Journal:   TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)   2004 , Volume 62 , Number 5; Page(s) 357 To 362.
 
Paper: 

IDENTIFICATION OF LEISHMANIA SPECIES FROM ATYPICAL LESIONS USING MONOCLONAL ANTIBODIES

 
 
Author(s):  SADEGHIAN G., HEJAZI SH., NILFROUSHZADEH M.A.
 
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Abstract: 

Background: Cutaneous Leishmaniasis is a parasitic infection caused by different species of Leishmania. Some atypical forms of the disease were seen different from usual forms with respect to the course and appearance of disease. The aim of this study is to determine the Leishmania species, which are isolated from the mentioned forms.
Materials and Methods: A descriptive study was done to determine the leishmania species in patients with atypical forms of the disease. Patients selected by easy sampling tested by direct smear and cultured in NNN media. For mass production, the isolates were transferred to RPMI 1640 media which was supplemented by 20% FCS. The promastigotes were coated in plates and were characterized with specific monoclonalantibody and ELISA methods.
Results: Of 74 patients, L. major and L. tropica were found in 87.8% and 12.2% of them, respectively. Unusual lesions were seen to be satellite (40.54%), sporotrichoid (33.78%), chronic (9.45%), zosteriform (6.75%), verrucose (5.4%) and erysipeloeid form in 4.05% of the cases. 50 patients (67.56%) hadnt received any treatment before appearing atypical lesions. In contrast, 24 cases had undergone different treatments for cutaneous leishmaniasis. 36 patients (out of 74) were from urban area of which L. tropica and L. major were derived in 25% and 75% , respectively. The Others originated of rural ones have L. major in their lesions.
Conclusion: Isolated species were L.major and L.tropica. There was no link between atypical forms and isolated species. The most of the atypical forms were found on extremity. No significant relation between the previous therapy, its presence or absence and form of the lesion was seen. Both species were isolated from the urban area. But in the rural regions only L.major was seen.

 
Keyword(s): CUTANEOUS LEISHAMANIASIS, ATYPICAL FORM, L. MAJOR, L. TROPICA, MONOCLONAL ANTIBODIES, IDENTIFICATION
 
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