Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)   Summer 2001 , Volume 8 , Number 2 (SN 20); Page(s) 13 To 18.
 
Paper: 

DETECTION OF RESISTANT GENE TO METHICILLIN (MEC-A) IN PURULENT INFECTIONS RELATED TO STAPHYLOCOCCUS USING PCR

 
 
Author(s):  SADEGHIAN S.*
 
* Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences & Health Services, Hamadan, Iran
 
Abstract: 

The emergence of Staphylococcus strains resistant to methicillin occurred shortly after the introduction of methicillin into clinical use.
Resistance to methicillin in clinical isolates of Staphylococci has been explained by the production of a characteristic penicillin-binding protein (PBP), designated PBP2a, that has a decreased binding affinity for - lactam antibiotics, the gene mec-A encoding PBP2a was cloned and is distributed among methicillin- resistant but not methicillin-susceptible populations of S. aureus. Recently, high prevalence of methicillin resistance has been noticed in the clinical isolates of coagulase-negative Staphylococci (CNS) which, besides MRSA (Methicillin- Resistant -Staphylococcus aureus), also have been recognized as important nosocomial pathogens.
Detection of the mec-A using PCR may provide a sensitive method for identifying methicillin resistant Staphylococci.
In this study, overall 268 wound swabs in which 107 cases (40%) were Staphylococcus aureus and 10 cases (3.7%) were coagulase negative Staphylococcus and the rest of them were other microorganisms were examined .
After the extraction of DNA, PCR was carried out using specific primers. The results indicated that the mec-A was present in 24 cases (9%). In 8 cases (34%) Staphylococcus aureus,3 cases (12%) coagulase negative Staphylococci, the others were related to another microorgamisms that probably were contaminated by Staphylococcus species.

 
Keyword(s): GENE , MEC-A, METHICILLIN, POLYMERASE CHAIN REACTION, STAPHYLOCOCCAL INFECTIONS
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 80
 
Latest on Blog
Enter SID Blog