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Paper Information

Journal:   AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)   Winter 2002 , Volume 8 , Number 4 (SN 22); Page(s) 32 To 36.
 
Paper: 

THE EFFECT OF VOLATILE OILS AND VITAMIN C ON THE AFFINITY OF LDL TO ITS RECEPTOR: A MODEL FOR PREVENTION OF ATHEROSCLEROSIS

 
 
Author(s):  SAFARI M.R.*, ANI M., NADERI GH., ASGARI SEDIGHEH
 
* Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences & Health Services, Hamadan, Iran
 
Abstract: 

For several years, scientists tried to find antioxidant compounds that inhibit the low density lipoprotein (LDL) oxidation reaction but have no destructive effects. Vitamin C and vitamin E can inhibit LDL oxidation. Volatile oils possess herbal sources and because of lipophilic properties can enter LDL and their antioxidant effects have not been known. The aim of this study is possible use of herbal products such as volatile oils in preventing or treating atherosclerosis. Because the probable effects of volatile oils on the affinity of LDL to its receptor, this affinity can be influenced and the modification of LDL and atherogenesis be prevented.
In this study, antioxidant properties of various concentrations of three volatile oils include: P-cymol, Limonene and Thymol , and vitamin C on the affinity of natural-LDL and oxidized-LDL(with Cu+2) to its receptor in bovine adrenal tissue cells have been investigated in the presence of labeled-LDL with fluoresein isothiocyanate (FITC). First, after purification of LDL-solvent of normal serum and incubation it with these compounds, oxidation reaction performed and LDL-solvent was labeled with FITC, and then all solvents were added to the medium that contains adrenal tissue cells(with LDL-receptor). Finally, fluorescence intensity of supernatant of each sample was determined in spectrofluorometer. Also, the effects of the best concentration of vitamin C (obtained in this study) with each one of these compounds was studied.
Results showed that vitamin C in 100 micromolar concentration has the greatest effect on the increasing affinity of LDL to its receptor (for natural- LDL 18.5% and for oxidized-LDL 62%). Among the volatile oils used, "Thymol" had the best effect (for natural-LDL 27% and for oxidized-LDL 50%). In studying the effects of these three volatile oils with the best concentration of vitamin C (100 micromolar) , "Thymol" had the highest effect on this affinity.
These finding showed that some compounds probably can change the affinity of LDL to its receptor and thus may prevent formation of atherosclerosis.

 
Keyword(s): ASCORBIC ACID, ATHEROSCLEROSIS, LIPOPROTEINS, LDL, VOLATILE OIL
 
References: 
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