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Paper Information

Journal:   IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH   2002 , Volume - , Number 10; Page(s) 15 To 27.
 
Paper: 

OPTIMIZING DNA EXTRACTION PROCEDURE IN CASE OF AMYGDALUS SPP.

 
 
Author(s):  KADKHODAEI S., TABAEI AGHDAEI S.R.
 
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Abstract: 
A simple method was developed to extract sufficient amount of genomic DNA, from plant species containing high amount of secondary metabolites. Leaves of different Amygdalus species including A. scoparia, A. haussknechtii, A. lyciodes, A. elaegnifolia and A. commonis were used for DNA extraction. SDS (instead of CTAB or Sarkosile), NaCl (to remove polysaccharides), PVP (to remove polyphenolic compounds) were used in extracting buffer. Sodium chloride was used again for more polysaccharide removal and RNase to remove RNA. Also, extraction was repeated several times, using chloform: isoamyl- alchohol to abtain more purified DNA. Average yield of DNA, extracted with this procedure was 50 mg per 1 g leaf tissue (70mg/g and 30mg/g in young and old leaf tissues, respectively). Extracted DNAs showed successful reproducibility through PCR amplification. This method which is relatively inexpensive could be efficiently applied for other species of rosaceae or different plant families from which DNA extraction is cumbersome.
 
Keyword(s): DNA EXTRACTION, AMYGDALUS SPP., POLYSACCHARIDES, POLYPHENOLES
 
 
References: 
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Cite:
APA: Copy

KADKHODAEI, S., & TABAEI AGHDAEI, S. (2002). OPTIMIZING DNA EXTRACTION PROCEDURE IN CASE OF AMYGDALUS SPP.. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, -(10), 15-27. https://www.sid.ir/en/journal/ViewPaper.aspx?id=120255



Vancouver: Copy

KADKHODAEI S., TABAEI AGHDAEI S.R.. OPTIMIZING DNA EXTRACTION PROCEDURE IN CASE OF AMYGDALUS SPP.. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH. 2002 [cited 2021April20];-(10):15-27. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=120255



IEEE: Copy

KADKHODAEI, S., TABAEI AGHDAEI, S., 2002. OPTIMIZING DNA EXTRACTION PROCEDURE IN CASE OF AMYGDALUS SPP.. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, [online] -(10), pp.15-27. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=120255.



 
 
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