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Paper Information

Journal:   IRANIAN BIOMEDICAL JOURNAL   JANUARY 2008 , Volume 12 , Number 1; Page(s) 15 To 21.
 
Paper: 

RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA

 
 
Author(s):  MOUSAVI S.L.*, NAZARIAN SH., AMANI J., KARIMI RAHJERDI A.
 
* DEPT. OF BIOLOGY, COLLEGE OF BASIC SCIENCES, SHAHED UNIVERSITY, TEHRAN
 
Abstract: 

Background: The ability to sensitively detect Vibrio cholera with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The aim of this research is to evaluate the suitability of a PCR-enzyme-linked immunosorbent assay for sensitive and rapid detection of V. cholera O1. Methods: The 398-bp sequence of a gene that codes for the cholera toxin B subunit was amplified by PCR. The digoxigenin-labeled amplified products were coated on microplates and detected by ELISA. The PCR product was also hybridized with biotin labelled probe and detected by ELISA using streptavidin. Results and Conclusion: The specificity of the PCR was determined using 10 bacterial strains and 50 samples from south Iran. The detection limit was 0.5 pg of the genomic DNA and five bacterial cells. Adaptation of PCR into PCR-ELISA assay format facilitates specific and sensitive detection and diagnosis of human cholera disease. We conclude that this PCR-ELISA is a diagnostic method that specifically detects toxin genes in V. cholera O1 strains. It is more rapid and less cumbersome than other diagnostic methods for detection of toxicity in these strains.

 
Keyword(s): VIBRIO CHOLERA, PCR- ELISA, IMMUNOSORBENT ASSAY
 
 
References: 
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Citations: 
 
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APA: Copy

MOUSAVI, S., & NAZARIAN, S., & AMANI, J., & KARIMI RAHJERDI, A. (2008). RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA. IRANIAN BIOMEDICAL JOURNAL, 12(1), 15-21. https://www.sid.ir/en/journal/ViewPaper.aspx?id=100777



Vancouver: Copy

MOUSAVI S.L., NAZARIAN SH., AMANI J., KARIMI RAHJERDI A.. RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA. IRANIAN BIOMEDICAL JOURNAL. 2008 [cited 2021October26];12(1):15-21. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=100777



IEEE: Copy

MOUSAVI, S., NAZARIAN, S., AMANI, J., KARIMI RAHJERDI, A., 2008. RAPID SCREENING OF TOXIGENIC VIBRIO CHOLERAE O1 STRAINS FROM SOUTH IRAN BY PCR-ELISA. IRANIAN BIOMEDICAL JOURNAL, [online] 12(1), pp.15-21. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=100777.



 
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