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Paper Information

Journal:   JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)   WINTER 2007 , Volume 16 , Number 62/1 (BIOLOGY ISSUE); Page(s) 91 To 104.
 
Paper: 

THE INHIBITORY EFFECTS OF PLANT METHANOLIC EXTRACT OF DATURA STRAMONIUM L. AND LEAF EXPLANTS DUE TO CALLI AGAINST BACTERIA AND FUNGI

 
 
Author(s):  IRANBAKHSH A.*, EBADI M.
 
* BIOLOGY DEPARTMENT, GARMSAR BRANCH, ISLAMIC AZAD UNIVERSITY, GARMSAR, I.R.IRAN
 
Abstract: 

The study intends to investigate the effects of methanolic extract of root, stem and leaf of Datura stramonium L. in vegetative and generative phases on the growth process of four bacterial strains, (Escherichia coli, Pseudomonas aeruginosa, Staphylococus epidermidis, Bacillus subtilis) and four fungi strains (Fusarium semithectum, Fusarium colmorum, Ceratocystis ulmi, Rhizoctoina solani). In this research, methanol extract of flower, seed and leaf explants Callus was also treated. Liquid culture, Pour plate, punch, direct drop and disk paper methods was used. The result showed that: Inhibition effect of methanol extract of organogenesis and green calli due to leaf explants on the growth of B. subtilis (ATCC KXI-Al 15561) and S. epidermidis (ATCC 0-1-B29997) was seen (Inhibition zones 22mm and 23mm, respectively). Inhibition effect of methanol extract of clear callus on the growth of E. coli (ATCC W1485 25645) was seen (Inhibition zone 17mm). Effect of methanol extract of organogenesis calli on the growth of C. ulmi (ATCC 32731) was seen. Inhibition effect of methanol extract of green organogenesis calli on the growth of F. semithectum (ATCC 11599) was seen (Inhibition zones 17mm and 17mm, respectively). Effect of methanol extract of green and calli on the growth of F. colmorum (ATCC 15620) was seen. Inhibition effect ofmethanolic extract of stem and leaf in vegetative phase on the growth of B. subtilis was seen (analysis of punch method). Inhibition effect of methanolic extract of leaf and stem in vegetative phase (Inhibition zones 21mm and 21mm, respectively), stem and root in generative phase (with inhibition zone 20mm) and mature seed (Inhibition zones 17mm) on the growth of B. subtilis (Inhibition zone 21mm) was seen (direct drop method). Effect of methanolic extract of mature seed on the growth of P. aeruginosa (ATCC PA103- 29260) (Inhibition zone 21mm) was seen (direct drop method). Inhibitory effect of methanolic extract of root in vegetative phase and flower on the growth of R. solani was seen (Inhibition zones 20mm and 16mm, respectively). The results showed that the effective compound was atropine alkaloid.

 
Keyword(s): DATURA STRAMONIUM, BACTERIA, FUNGI, CALLUS, PLANT METHANOLIC EXTRACT, ATROPINE
 
References: 
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