APPLICATION OF MATRIX SOLID-PHASE DISPERSION–HPLC METHOD TO DETERMINE ELLAGIC ACID IN POMEGRANATE PEEL

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NAZARI SERENJEHAND F.; HASHEMI P.

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Paper Information

Seminar: IRANIAN CHEMISTRY CONGRESS
Year:2013 | Issue:16

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Title

APPLICATION OF MATRIX SOLID-PHASE DISPERSION–HPLC METHOD TO DETERMINE ELLAGIC ACID IN POMEGRANATE PEEL

Author(s)

NAZARI SERENJEHAND F. | HASHEMI P. | Issue Writer Certificate

Keywords

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Abstract

POLYPHENOLIC COMPOUNDS ARE WIDELY DISTRIBUTED IN THE VEGETABLE KINGDOM AND ARE OFTEN ENCOUNTERED IN OUR DAILY LIVES, BEING CONTAINED IN TEA, POMEGRANATE AND VARIOUS MEDICINAL PLANTS. AMONG THEM, ELLAGIC ACID (EA) HAS BEEN FOUND TO HAVE ANTIMUTAGENIC, ANTIVIRAL, WHITENING OF THE SKIN AND ANTIOXIDATIVE PROPERTIES.MATRIX SOLID-PHASE DISPERSION (MSPD) IS A VALUABLE TOOL FOR EXTRACTING VARIOUS ANALYTES FROM FOODSTUFFS. IN MSPD CLEAN-UP IS CARRIED OUT SIMULTANEOUSLY WITH, GENERALLY, GOOD RECOVERIES AND PRECISION. IN THIS STUDY, AN EFFICIENT METHOD WITH MSPD USING C18 AS SORBENT WAS DEVELOPED FOR THE ANALYSIS OF ELLAGIC ACID IN POMEGRANATE PEEL BY HIGHPERFORMANCE LIQUID CHROMATOGRAPHY (HPLC).SAMPLE WAS BLENDED WITH 0.1 G OF C18 AND THEN 40 ML DICHLOROMETHANE WAS ADDED AS DISPERSER. AFTER FINISHING THE MSPD BLENDING PROCESS, THE MIXTURE WAS PACKED INTO A CARTRIDGE. THEN, THE MSPD COLUMN WALLS WERE WASHED WITH N-HEXANE AND THE COLUMN PACKING DRIED WITH A STRONG STREAM OF AIR. THE COLUMN WAS ELUTED WITH METHANOL AND THIS SOLUTION WAS INJECTED INTO THE HPLC SYSTEM.SIX REPLICATED ANALYSIS AT THE OPTIMIZED CONDITIONS, RESULTED IN A RECOVERY OF 90.9% AND AN RSD OF 6.6%. THE MSPD METHOD IS FAST, EASY, ACCURATE AND RELIABLE. THE PROPOSED METHOD WAS SUCCESSFULLY APPLIED TO THE EFFICIENT DETERMINATION OF ELLAGIC ACID IN PEELS FROM SEVERAL POMEGRANATE VARIETIES.

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APA: Copy

NAZARI SERENJEHAND, F., & HASHEMI, P.. (2013). APPLICATION OF MATRIX SOLID-PHASE DISPERSION–HPLC METHOD TO DETERMINE ELLAGIC ACID IN POMEGRANATE PEEL. IRANIAN CHEMISTRY CONGRESS. SID. https://sid.ir/paper/913521/en

Vancouver: Copy

NAZARI SERENJEHAND F., HASHEMI P.. APPLICATION OF MATRIX SOLID-PHASE DISPERSION–HPLC METHOD TO DETERMINE ELLAGIC ACID IN POMEGRANATE PEEL. 2013. Available from: https://sid.ir/paper/913521/en

IEEE: Copy

F. NAZARI SERENJEHAND, and P. HASHEMI, “APPLICATION OF MATRIX SOLID-PHASE DISPERSION–HPLC METHOD TO DETERMINE ELLAGIC ACID IN POMEGRANATE PEEL,” presented at the IRANIAN CHEMISTRY CONGRESS. 2013, [Online]. Available: https://sid.ir/paper/913521/en

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