SELECTING OPTIMAL CULTIVATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS FOR CLINICAL CELL THERAPY (POSTER PRESENTATION)

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FROUZAN NIA S.K.; POURRAJAB F.; TABATABAEE S.A.; SAREBAN M.T.

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Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2011 | Volume:13 | Issue:SUPPLEMENT 3 (7TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY) Page(s): 32-32

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Title

SELECTING OPTIMAL CULTIVATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS FOR CLINICAL CELL THERAPY (POSTER PRESENTATION)

Author(s)

FROUZAN NIA S.K. | POURRAJAB F. | TABATABAEE S.A. | SAREBAN M.T.

Keywords

ISOLATION

EXPANSION

CONDITIONED- MEDIUM

MESENCHYMAL STROMAL CELLS

Abstract

Objective: Clinical application of human MESENCHYMAL STROMAL CELLS (hMSCs) and successful stem cell therapy requires their EXPANSION to be safe and rapid. We aimed to evaluate the alternatives of ISOLATION and cultivation methods of hMSC to develop a protocol which would shorten the EXPANSION time and avoid multiple passaging to reduce contamination risks.Materials and Methods: First, we have compared the EXPANSION of the Ficoll gradient - isolated and non- isolated cell suspensions in alpha-MEM and DMEM medium, separately. Then, co-culture and MSCs-conditioned medium was evaluated for EXPANSION and proliferation capacity of the cells.Results: When the Ficoll gradient density ISOLATION was used and non-adherent cells were discarded, both media were comparable whereas, significantly more hMSCs were obtained from non-isolated bone marrow cultures in DMEM medium. Furthermore, the numbers of hMSCs were significantly higher when non-adherent cells or supernatants were not discarded. However, flask-culture system seemed more favorable and led to increase of hMSCs after two weeks of cultivation compared to 100 mm plate cultures.Conclusion: Our experiment thus, admits previous observations and leads to a protocol for more effective hMSC production which can be used in cell therapy.

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APA: Copy

FROUZAN NIA, S.K., POURRAJAB, F., TABATABAEE, S.A., & SAREBAN, M.T.. (2011). SELECTING OPTIMAL CULTIVATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS FOR CLINICAL CELL THERAPY (POSTER PRESENTATION). CELL JOURNAL (YAKHTEH), 13(SUPPLEMENT 3 (7TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY)), 32-32. SID. https://sid.ir/paper/577117/en

Vancouver: Copy

FROUZAN NIA S.K., POURRAJAB F., TABATABAEE S.A., SAREBAN M.T.. SELECTING OPTIMAL CULTIVATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS FOR CLINICAL CELL THERAPY (POSTER PRESENTATION). CELL JOURNAL (YAKHTEH)[Internet]. 2011;13(SUPPLEMENT 3 (7TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY)):32-32. Available from: https://sid.ir/paper/577117/en

IEEE: Copy

S.K. FROUZAN NIA, F. POURRAJAB, S.A. TABATABAEE, and M.T. SAREBAN, “SELECTING OPTIMAL CULTIVATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS FOR CLINICAL CELL THERAPY (POSTER PRESENTATION),” CELL JOURNAL (YAKHTEH), vol. 13, no. SUPPLEMENT 3 (7TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY), pp. 32–32, 2011, [Online]. Available: https://sid.ir/paper/577117/en

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