Fragmentation is occurred in more than 50% of human embryos. This phenomenon is a field of active research. By, far, several investigations were conducted with the aim of finding reliable methods to prevent or reduced the unwanted effects of fragments on intact blastomeres and then improve the growth and development of such embryos after transferring to uterus. The information is rare regarding in vitro developmental potential of such embryo. In present study, at first the impact of each fragmentation pattern on in vitro embryo growth and development were determined, then the potential of two methods, co-culture and fragment removal, to improve growth and development of fragmented embryos in vitro evaluated. For this purpose as a first step, two days human embryos were allocated to four grade according their fragmentation pattern (grade I were embryos with less than 5% fragmentation and the fragments located mainly under zona pellucida, the embryos with 20% diffused fragmentation considered as grade II, and those with 35-50% fragmentation were noted as grade III, such embryos had large and diffuse fragments. Finally the embryos with more than 50% fragmentation and necrotic appearance viewed as grade IV embryos). In second step, three groups including control (rS1, rS2), Experiment I (co culture with Vero cell) and Experiment II (fragment removal) were defined and embryos from all grades were cultured on each group (328, 304 and 197 embryos respectively) and their growth and development were daily recorded during 4 day culture period. In the end of cultivation, blastocysts developed in each group were scored according to the kind of TE, ICM, and expansion rate. In addition, the area of each blastocyst was measured in mm for further evaluation. Also each blastocyst was stained differentially for counting the cell number and evaluating embryo quality. To count the number of dead cells, each embryo was stained using TUNEL, method and finally the results of each group were analyzed and compared by c2, ANOVA or Kruskal-wallis. Our results showed that the embryos with high fragmentation rate had less developmental rate and quality, less area and cell number, and high dead cell compared to embryos with low fragmentation rate. Using fragment removal or co-culture systems results in significant improvement of embryos developmental rate and blastocyst quality in all grades. More specially, embryos from grades III and IV showed significant reduction of dead cells number after cultivating in co culture system or using fragment removal compared to those from control groups.