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Paper Information

Journal:   ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH   WINTER 2007 , Volume 4 , Number 4 (SERIAL NUMBER 16); Page(s) 1005 To 1009.
 
Paper: 

CONSIDERATION SENSITIVITY OF PCR-RIBOTYPING METHOD IN IDENTIFICATION SALMONELLA SPP ISOLATES FROM PATIENTS WITH DIARRHEA

 
 
Author(s):  MEHRVAR N.*, AKHAVAN SEPAHI A., AZIMIRAD M., MIRSAIDI K., ZALI MOHAMMAD REZA
 
* AZAD UNIVERSITY OF MEDICAL SCIENCES, DEPARTMENT OF MICROBIOLOGY
 
Abstract: 

Background: Salmonella is the most important diarrheagenic pathogens, which can cause food borne disease where the main route of transmission among human is through contaminated meat and poultry foods. Its symptoms can be diarrhea, fever, vomiting and sometimes bloody diarrhea. For its importance, it is essential to be identified and characterized by more precise methods such as molecular techniques. The aim of this study was to consider sensitivity of PCR-Ribotyping method for identification of Salmonella spp.
Materials and methods: In this study our samples were Salmonella strains, which were isolated from patients with diarrhea. Their DNA was extracted by phenol! Chloroform method. We did PCR-Ribotyping method with P1, P2 primers for 16S-23SrRNA gene. At last PCR-products run on 1.8% agarose gel in 120V for 90 min. the analysis was done after Ethidium bromide staining.
Results: All the 40 strains containing paratyphi A, B, C and 0 serotypes also, serotype typhi contained 5 bands ranging 700 to 2500 bp.
Conclusions: According to the results we can say that PCR-Ribotyping method has the highest sensitivity for identification of genus Salmonella but it is not suitable for Serotyping of Salmonella strains.

 
Keyword(s): FOOD- BORNE DISEASE, PCR-RIBOTYPING, SALMONELLAE SPP
 
References: 
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