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Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ)   2006 , Volume 15 , Number 4; Page(s) 239 To 245.
 
Paper: 

EFFECT OF SUCROSE AND GROWTH REGULATORS ON SOYBEAN SENSITIVITY TO GLYPHOSATE AND METRIBUZIN HERBICIDES AND DETERMINATION OF IN VITRO SENSITIVITY RANGE

 
 
Author(s):  SHARIFI A.*, BAGHERI A.R., VESAL S.R.
 
* DEPARTMENT OF WEED SCIENCE, FACULTY OF AGRICULTURE, UNIVERSITY OF FERDOWSI MASHHAD, MASHHAD, IRAN
 
Abstract: 

In Vitro culture of plants is one of the excellent tools for screening resistant lines to different stresses. The first step for the production of herbicide resistant lines is introduction of a suitable medium for screening appropriate resistant cell lines. In the medium compounds, sucrose and growth regulators are the most important compounds that are effective for herbicide effects. For this reason, in the first experiment effect of sucrose rates and kind of hormone compounds in the medium on sensitivity of explants to glyphosate and metribuzin, in order to introduce suitable medium, were investigated for screening appropriate resistant ceil lines. In the second experiment, the in vitro sensitivity range of soybean to these herbicides was determined in order to introduce suitable dosage for screening of resistant cell lines. Since this herbicide affect the photosynthetic system, result of the first experiment indicated that the hormone compounds of medium that stimulate chlorophyll production in the explant, will have suitable screening resistant cell lines and in this experiment, the medium with BA stimulated chlorophyll production and the medium with 2, 4-D and kinetin prevented chlorophyll production in the explants. Also sucrose as a supplying source of carbon and energy necessary for explants, affected the sensitivity of explants to these herbicides and reduced the effect of these herbicides and, therefore, one percent sucrose was recommended. In the second experiment, sensitivity range of soybean to metribuzin and glyphosate were 1-100 mgai/l of these herbicides and in order to screen the resistant cell lines, rates of 111.4 and 31.7 mgai/l for metribuzin and glyphosate were recommended, respectively.

 
Keyword(s): 2, 4-D, BA, GLYPHOSATE, IN VITRO CULTURE, METRIBUZIN, SOMACLONAL VARIATION, SOYBEAN
 
References: 
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