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Paper Information

Journal:   IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE)   2006 , Volume 25 , Number 4; Page(s) 73 To 76.
 
Paper: 

DETERMINATION OF JUGLONE (5-HYDROXY 1,4-NAPHTHOQUINONE) IN PTEROCARYA FRAXINIFOLIA BY RP-HPLC

 
 
Author(s):  HAJ MOHAMMADI M.R.*, KAMEL K.
 
* DEPARTMENT OF CHEMISTRY, UNIVERSITY OF MAZANDARAN, BABOLSAR, I.R. IRAN
 
Abstract: 

A method for determination of juglone in leaves and hulls of Pterocarya fraxinifolia was developed based on RP-HPLC. This compound was extracted from leaves and hulls by chloroform refluxing about 2 hours. The chloroform was evaporated under low pressure at   50 ºC to produce a dry residue. The remained materials were dissolved in acetonitrile to be used for determination of this compound. Appropriate conditions for RP-HPLC were determined using standard of juglone. These conditions include, acetonitrile percentage in mobile phase, pH of mobile phase (phosphate buffer) and temperature of column, which were 50%, 4 and 30ºC, respectively. Juglone concentration was determined using standard addition method. The concentrations of juglone were 2.15, 2.74,1.77,1.12and 0.34 g in 100 g of dry leaves in May, June, July, August and September 2001, respectively. The content of juglone in hulls was 0.44 g in 100 g of dry hulls in May.

 
Keyword(s): PTEROCARYA FRAXINIFOLIA, JUGLONE, NAPHTHOQUINONE, RP-HPLC METHOD
 
References: 
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