Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   Spring-Summer 2005 , Volume 23 , Number 76-77 (Special English Edition); Page(s) 64 To 71.
 
Paper: 

DIAGNOSTIC VALUE OF AGNOR STAINING IN THE DIFFERENTIATION BETWEEN HODGKIN'S/NON-HODGKIN'S LYMPHOMA AND REACTIVE LYMPHOID LESIONS

 
 
Author(s):  KATEBI M.*, OMIDI A.A, SHARIFY N.
 
* Department of Pathology, Ghaem Hospital Mashhad University of Medical Sciences, Mashhad, Iran
 
Abstract: 

INTRODUCTION: AgNOR staining, as a cell proliferation marker, has been used in the diagnosis, differentiation and prognosis assessment of different malignant and benign neoplasms. This study was performed to evaluate the application of AgNOR staining technique in differentiation of malignant and benign lymphoid lesions and also in classification of different types of lymphoma. In this technique, the numbers and mean maximum diameter of dots in cell nuclei are measured via optical microscopy.
METHODS: In this study, 100 samples of benign and malignant lymphoid lesions were collected from the Department of pathology of Ghaem hospital. of Mashhad Medical School between 1999 and 2002. 3-µm paraffin sections were prepared from samples fixed in 10% formalin, and the quality and quantity of nucleolar organizer regions (NORs) were evaluated by means of AgNOR staining method. AgNOR staining was performed by modified Crocker technique. The number of AgNOR dots on 100 cells in each slide was counted randomly by optical microscope, using oil immersion lens with x1000 magnification. Maximum diameter (Dmax) of AgNOR dots was measured by morphometric interactive image analysis system (Carl-Zeiss) on photographic pictures taken by digital camera microscope on final magnification of x4000. The number of mean AgNOR count, the percentage of cells with >3 and
5 dots and Dmax of AgNORs was evaluated for correlation and significance of differences.
RESULTS: It was observed that the mean counts of AgNOR dots and the percentage of cells with >3 and
5 dots were significantly increased in reactive nodes, Hodgkin's lymphoma and Non-Hodgkin's lymphoma respectively. There were increasing values for the mean counts of AgNOR dots and the percentage of cells with >3 and 5 dots, from low grade to intermediate and finally high-grade Non-Hodgkin's lymphomas. The order was reverse for Dmax of AgNORs in the above samples (greatest values in reactive nodes and smaller value in high-grade Non-Hodgkin's lymphomas).
Also, changes in the parameters of Dmax values were according to the prognostic classification of Classic Hodgkin's lymphoma. In all cases, the differences between values were highly significant (P<0.001) and strong correlation was observed between parameters.
DISCUSSION: It seems that AgNOR staining is a valuable and simple method for differentiation of neoplastic and reactive lymphoid lesions, classification of Non-Hodgkin's lymphomas and prediction of prognosis.

 
Keyword(s): AGNOR , NON-HODGKIN'S LYMPHOMA, HODGKIN'S LYMPHOMA, REACTIVE LYMPHOID LESIONS
 
References: 
  • ندارد
 
  Yearly Visit 54
 
Latest on Blog
Enter SID Blog