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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   Spring-Summer 2005 , Volume 23 , Number 76-77 (Special English Edition); Page(s) 1 To 5.
 
Paper: 

COMPARISON OF THE OUTCOME OF EOSIN-NIGROSIN, HYPO-OSMOTIC SWELLING TEST AND SPERM MTT VIABILITY ASSAY IN ASTHENOSPERMIA AND NORMOSPERMIA

 
 
Author(s):  ABOUTORABI R.*, NASRESFAHANI MOHAMMAD HOSSEIN, RAZAVI SH.
 
* Department of Anatomical sciences, Isfahan University of Medical Sciences Royan Institute, Iran University of Medical Sciences, Isfahan, Iran
 
Abstract: 

INTRODUCTION: Sperm MTT viability assay is a new method for assessment of percentage of viable sperms in semen samples. The objective of this study was to evaluate the capability of sperm MTT viability assay, in comparison with eosin-nigrosin and hypo-osmotic swelling tests, in severe and moderate asthenospermia and normospermia.
METHODS: MTT, E&N and HOST were carried out simultaneously on 37 human semen samples. Nine semen samples had less than 5% motility, eleven semen samples had 10-25% motility and seventeen semen samples had more than 50% motility. Two paired t-tests were conducted using SPSS, statistical software.
RESULTS: No significant differences were observed between the above 3 sperm viability tests between the aforementioned patients.
DISCUSSION: Sperm MTT viability assay can be used as a diagnostic test for differentiating viable from non-viable sperms in all of these three groups.

 
Keyword(s): HUMAN SPERM, MTT, ASTHENOSPERMIA, NORMOSPERMIA
 
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