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Paper Information

Journal:   MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI)   February 2002 , Volume 15 , Number 4; Page(s) 219 To 225.
 
Paper: 

TRANSFORMING GROWTH FACTOR ß2 UP-REGULATES GM-CSF GENE IN HUMAN BLADDER CARCINOMA CELL LINE HTB 5637

 
 
Author(s):  SOHEYLI Z.S., GOLIAEI B.*
 
* Laboratory of Biophysics and Molecular Biology, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, I.R. Iran
 
Abstract: 

Transforming growth factor betas are multifunctional polypeptides in the cytokine superfamily. They have a growth inhibitory role on hemopoietic progenitor cells in semisolid colony assay as well as in long-term bone-marrow culture. TGF-ß2 represses stromal cells, stem cell factor gene transcription, and decreases the stability of c-kit transcripts in hemopoietic cells. TGF- ß also modulates GM-CSF production from human lymphocytes. The present study reveals the TGF- ß2 role in production of GM-CSF in HTB 5637, human bladder carcinoma cell line. HTB 5637 cells were treated with 5 ng/mL of human TGF- ß2 viable cells were counted and GM-CSF concentrationwas determined. No antiproliferate activity of TGF- ß2 on HTB 5637 cell line was observed. Biological assay showed increased levels of GM-CSF in the supernatant of cultured cells. However this increase was lower than that expected from ELISA. Since TGF- ß may be an active suppressor factor regulating hemopoiesis, it seems that some inhibitory factor(s) may be produced (increased) in response to TGF- ß2 treatment.  It has been shown that GM-CSF mRNA content from HTB 5637 cell line is very stable and this stabilization is translational dependent. Using Slot blot and Northern blot analysis, we determined that TGF- ß2 upregulated GM-CSF gene expression in HTB 5637 cell line. The results suggest that TGF- ß2 upregulates the production of GM-CSF gene at the transcriptional level.

 

 

 

 
Keyword(s): TGF-ß, GM-CSF, HTB-5637, BLADDER CARCINOMA
 
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