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Paper Information

Journal:   DANESHVAR MEDICINE   JUNE-JULY 2005 , Volume 12 , Number 57; Page(s) 21 To 26.
 
Paper: 

ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA

 
 
Author(s):  POUPAK B., POURFATH ELAH A.A., YAHYAVI S.H., NAJMABADI H., MORTAZAVI YOUSEF, VOSOUGH PARVANEH, IZADYAR MINA, SHAHGHOLI E., BAHOUSH GH.R., HAGHNEZHAD DOUSHANLOU F.
 
* 
 
Abstract: 

Introduction: Rearrangement of V, 0 & J segments of immunoglobulin heavy chain's gene with inserted or deleted nucleotides between rearranged segments make unique hyper variable regions (COR - 3).These regions can be used for evaluation of clonality & minimal residual disease using PCR.

Method: 71 children with diagnosis of B - precursor acute lymphoblastic leukemia who were admitted in Ali Asghar & Children Medical Center Hospitals were studied. After DNA extraction of mononuclear cells of bone marrow aspirates, PCR was done using consensus primers for amplification of COR - 3 region. PCR products were analyzed after heteroduplex analysis

using polyacrylamide gel electrophoresis & silver staining.

Results: Eighty two percent of patients showed clonal rearrangement. Sixty nine, twenty - four and seven percent of patients showed monoclonal, biclonal & oligoclonal patterns, respectively.

Conclusion: As a result of acceptable sensitivity assay, evaluation of MRD is possible.

 

 

 

 
Keyword(s): ACUTE LYMPHOBLASTIC LEUKEMIA, CLONALITY, MINIMAL RESIDUAL DISEASE (MRD), POLYMERASE CHAIN REACTION
 
References: 
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