Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   DANESHVAR MEDICINE   JUNE-JULY 2005 , Volume 12 , Number 57; Page(s) 21 To 26.
 
Paper: 

ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA

 
 
Author(s):  POUPAK B., POURFATH ELAH A.A., YAHYAVI S.H., NAJMABADI H., MORTAZAVI YOUSEF, VOSOUGH PARVANEH, IZADYAR MINA, SHAHGHOLI E., BAHOUSH GH.R., HAGHNEZHAD DOUSHANLOU F.
 
* 
 
Abstract: 

Introduction: Rearrangement of V, 0 & J segments of immunoglobulin heavy chain's gene with inserted or deleted nucleotides between rearranged segments make unique hyper variable regions (COR - 3).These regions can be used for evaluation of clonality & minimal residual disease using PCR.

Method: 71 children with diagnosis of B - precursor acute lymphoblastic leukemia who were admitted in Ali Asghar & Children Medical Center Hospitals were studied. After DNA extraction of mononuclear cells of bone marrow aspirates, PCR was done using consensus primers for amplification of COR - 3 region. PCR products were analyzed after heteroduplex analysis

using polyacrylamide gel electrophoresis & silver staining.

Results: Eighty two percent of patients showed clonal rearrangement. Sixty nine, twenty - four and seven percent of patients showed monoclonal, biclonal & oligoclonal patterns, respectively.

Conclusion: As a result of acceptable sensitivity assay, evaluation of MRD is possible.

 

 

 

 
Keyword(s): ACUTE LYMPHOBLASTIC LEUKEMIA, CLONALITY, MINIMAL RESIDUAL DISEASE (MRD), POLYMERASE CHAIN REACTION
 
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
  • Not Registered.
 
+ Click to Cite.
APA: Copy

POUPAK, B., & POURFATH ELAH, A., & YAHYAVI, S., & NAJMABADI, H., & MORTAZAVI, Y., & VOSOUGH, P., & IZADYAR, M., & SHAHGHOLI, E., & BAHOUSH, G., & HAGHNEZHAD DOUSHANLOU, F. (2005). ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA. DANESHVAR MEDICINE, 12(57), 21-26. https://www.sid.ir/en/journal/ViewPaper.aspx?id=47764



Vancouver: Copy

POUPAK B., POURFATH ELAH A.A., YAHYAVI S.H., NAJMABADI H., MORTAZAVI YOUSEF, VOSOUGH PARVANEH, IZADYAR MINA, SHAHGHOLI E., BAHOUSH GH.R., HAGHNEZHAD DOUSHANLOU F.. ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA. DANESHVAR MEDICINE. 2005 [cited 2021October24];12(57):21-26. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=47764



IEEE: Copy

POUPAK, B., POURFATH ELAH, A., YAHYAVI, S., NAJMABADI, H., MORTAZAVI, Y., VOSOUGH, P., IZADYAR, M., SHAHGHOLI, E., BAHOUSH, G., HAGHNEZHAD DOUSHANLOU, F., 2005. ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA. DANESHVAR MEDICINE, [online] 12(57), pp.21-26. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=47764.



 
 
Persian Abstract Yearly Visit 57
 
 
Latest on Blog
Enter SID Blog