Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)   Fall 2000 , Volume 3 , Number 3 (12); Page(s) 29 To 34.
 
Paper: 

EVALUATION OF PLOIDY STATUS IN PATIENTS WITH LYMPHOMA (NON-HODGKIN AND HODGKIN) BY FLOW CYTOMETRY, WITH PARAFFIN-EMBEDDED TISSUE

 
 
Author(s):  KARIMI FARIDEH, FARHADI LANGEROUDI MOHAMMAD, POURFATH ELAH A.A., HOSSEINI H.
 
* 
 
Abstract: 

DNA analysis by flow cytometry is one of novel techniques recently introduce of utilize in routine clinical diagnostic laboratories, Generally, there is an overall agreement between DNA status and chromosomal content of cells, so with DNA analysis especially in neoplastic diseases, one could find the ploidy feature and number of cells which are in synthetic phase (S-phase fraction) and also cellular kinetics in selected neoplasia, which are characteristic findings and factors that could ve used as prognostic markers for predicting clinical behaviour and monitoring response to treatment protocol.

In this study, we measured DNA content in eighty eight (88) malignant lymphopreliferation diseases including 58 NHL and 30; HD diagnosed histopathologyically, by flow cytometry using Hedley method, Also immunohistochemical staining for proper immunophenotyping done in selected cases with ABC method with MoAb's. Aneuploidy were 38% in NHL and 47% in HD patients. This occurrence was unrelated to other parameters used as, Age, turner location and histologic subtypes (P>0.05).

S-phase fraction (SPF) measurement calculated as percentage of cellw in active phase and proligeration index determinations reveal higher frequency of SPF (PI) within Iranian patients rather than reported Western patients. This finding can account for lower survival rate and poor response to chemotherapy and (or radiotherapeutic regimen used in Iranian patients, but also other parameters in this regard also should be considered simultaneously.

With increasement in SPF% and PI% more aggression in tumor clinical behaviour with poor response to treatment and so poor prognosis result, So these patients, Finally as an important independent prognostic factors, DNA analysis and SPF% and PI% determination could be used if available and further studied especially those follow-ups that consider survival rate and ultimate clinical behaviour in patients with different values in DNA analysis.

 

 

 

 

 
Keyword(s): LYMPHOMA, DNA ANALYSIS, FLOW CYTOMETRY, SPF%, CELL PROLIFERATION
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 58
 
Latest on Blog
Enter SID Blog