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Paper Information

Journal:   JOURNAL OF MICROBIAL WORLD   JUNE 2015 , Volume 8 , Number 2 (23); Page(s) 130 To 138.
 
Paper: 

OPTIMIZATION OF CAROTENOID PIGMENT PRODUCTION BY NATIVE STRAINS HALOARCHAEA LIPOLITICAL

 
 
Author(s):  MOHAMMADI MASOMEH, POURBABAI AHMAD ALI*, JAVADI ALI
 
* DEPARTMENT OF MICROBIOLOGY, QOM BRANCH, ISLAMIC AZAD UNIVERSITY, QOM, IRAN
 
Abstract: 

Background & Objectives: The halophilic Archaea are strong sources of carotenoid pigments. These compounds are used in the pharmaceutical industries and fisheries due to their antioxidant, anti-tumor and pigmentation properties. This study was aimed to increase the production of pigment Carotenoids by the strains Haloarchaea lipolitical.
Materials & Methods: This experimental study was performed on H. lipolitical, isolated previously from water sources located at Bushehr. Effects of several factors, temperature, pH, carbon sources, nitrogen sources, different percentages of sodium chloride ions and the impact of heavy metals were compared with the standard curve to optimize the pigment production in H. lipolitical.
Results: Statistical analyzes showed the optimal amount of carotenoid pigment production conditions at a temperature 35
oC for 88 hours (39.3 mg), pH 8 for 120 hours (15.77 mg), casein peptone nitrogen source and yeast extract for 72 hours (13.28 mg), NaCl 16% for 96 hours (15.77 mg), starch carbon source for 96 hours (4.44mg) and the presence of manganese sulfate for 120 hours (59 mg).
Conclusion: Due to non-pathogenicity of Archaea, the availability of suitable sources for their growth and high levels of salt in the culture, this strain can be used for the production of carotenoids in various industries.

 
Keyword(s): CAROTENOIED, HALOPHILIC ARCHAEA, OPTIMIZATION
 
References: 
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