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Paper Information

Journal:   MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS   2014 , Volume 3 , Number SUPPL. (1); Page(s) 271 To 271.
 
Paper: 

IMMOBILIZATION OF LACTOPEROXIDASE ONTO COPPER NANOPARTICLE

 
 
Author(s):  SAKHAEI FARIBA*, SAMSAM SHARIAT SEYED ZIYAE ALDIN
 
* DEPARTMENT OF CLINICAL BIOCHEMISTRY, SCHOOL OF PHARMACY, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES
 
Abstract: 

Lactoperoxidase (EC.1.11.1.7), an enzyme of peroxidase family, is one of the most abundant bovine milk enzymes in which enter the whey in the process of cheese production. The lactoperoxidase enzyme has been of great interest due to its valuable properties, which can be widely used in medical field as well as in food fields. Recently, some studies have revealed that thermostability of the enzyme is not appropriate to use the enzyme in industry. The purpose of the present study was to immobilization of lactoperoxidase onto copper nanoparticles, and compare the thermostability of the native and the immobilized enzyme. Lactoperoxidase was purified from whey, a by-product in cheese industry by cation exchange and gel filtration chromatography .Then, purified enzyme was immobilized on the surface of copper nanoparticles by the method of Link et al. The immobilized enzyme displayed a similar pH- dependence behavior and an enhanced thermostability up to 56% compared to the native enzyme. Copper nanoparticles can be considered as an appropriate support for improvement of thermostability of the peroxidase enzymes family.

 
Keyword(s): IMMOBILIZATION, COPPER NANOPARTICLE, LACTOPEROXIDASE, THERMOSTABILITY, PEROXIDASE
 
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