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Paper Information

Journal:   THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES   Winter 2006 , Volume 16 , Number 4; Page(s) 205 To 211.
 
Paper: 

THE EFFECT OF VITRIFICATION ON APOPTOSIS OF SPERMATOZOA IN FERTILE AND SUBFERTILE MEN

 
 
Author(s):  PEYROVI T.*, KARIMIPOUR M., SOLAEYMANIRAD J., GHAFARI M.
 
* Medical Faculty, Urmia Medical Sciences University
 
Abstract: 

Background & Aims: Phospholipids are distributed asymmetrically between inner and outer leaflets of the plasma membrane of live cells. During early apoptosis this asymmetry is disrupted and phosphatidylserine becomes exposed on the outside surface of the plasma membrane. There is little information about the effects of vitrification on apoptosis. The aim of the present study is to evaluate the effect of vitrification on apoptosis of subfertile and fertile men. Materials and Methods: In this study, semen samples were collected from subfertile (n=20) and fertile men (n=10) after 48h abstinence of intercourse. After semen analysis according to WHO criteria, each semen sample was divided into two portions. First portion was assessed by Annexin V-flous staining kit for showing apoptosis in subfertile and fertile and second portion was assessed after vitrification-thawing.  Results were analyzed by paired t-test and Independent t-test.
Results: After vitrification-thawing, mean percentage of apoptotic spermatozoa has increased 3 and 6 times in subfertile and fertile men, respectively. This difference is significant.
Discussion: Vitrification-thawing could disrupted membrane asymmetry and caused apoptosis. Therefore, it causes reduction of functional spermatozoa in access of Assisted Reprouction Technologies (ART).

 

 

 
Keyword(s): APOPTOSIS, VITRIFICATION, THAWING, HUMAN SPERMATOZOA, FERTILE AND SUBFERTILE MEN
 
References: 
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