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Paper Information

Journal:   THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES   Winter 2006 , Volume 16 , Number 4; Page(s) 235 To 240.
 
Paper: 

IMMUNIZATION OF MICE WITH SONICATED ANTIGEN AGAINST HELICOBACTER FELLS AND COMPARING THE EMERGED IMMUNE RESPONSE WITH A NON IMMUNE H. FELLS INFECTED MICE

 
 
Author(s):  MORSHEDI A.*, MOHAMMADI M., AHMADI ASHTIANI H.R.
 
* Pathobiology Department, College of Veterinary, Urmia University, Urmia, Iran
 
Abstract: 

Background & Aims: The role of immunization of mice with sonicated antigen in protection of Helicobacter induced gastritis and invitro survey of lymphocyte response in immunized/ challenged mice in compare with infected mice was studied.
Materials & Methods: Two groups of mice were immunized with 3 mg of sonicated antigen and along with the 2 groups of naive (non-immunized) mice were challenged by alive H.felis. The lymphocyte proliferation index, were determined by dividing the counts obtained from Ag-stimulated cells by counting from unstimulated cell/well. The assay of IFN- γ and anti-H.felis antibodies were done by sandwich ELISA.
Results: Eight weeks after challenge, the lymphocyte stimulation index in infected and Immunized/challenge mice were respectively 45&25.lFN- γ production due to Immune/challenge and infected mice were respectively 300 and 170 Picogram/ml. The antibodies titer of Immune/challenge and infected mice obtained 1:8000 and 1:500 respectively.
Discussion: The data showed that cellular response of immunized mice were significantly higher (p<0.05) than infected mice, early after challenge. Whereas, declined within 8 weeks in Immunized mice in comparison with infected mice. On the other hand, the higher production of IFN- γ and anti-H. felis antibody by immunized mice, compared with infected mice, suggest that specific response, mediated by Th1 and then Th2 (via antibody production) play a role against Helicobacter-induced gastritis.

 

 

 
Keyword(s): HELICOBACTER FELIS, GASTRITIS, IMMUNIZATION, MICE
 
References: 
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