Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF FISHERIES SCIENCES   2012 , Volume 11 , Number 2; Page(s) 415 To 423.
 
Paper: 

IDENTIFICATION OF BESTER HYBRIDS (FEMALE HUSO HUSO LINNAEUS, 1758 AND MALE STERLET ACIPENSER RUTHENUS LINNAEUS, 1758) USING AFLP MOLECULAR TECHNIQUE

 
 
Author(s):  YARMOHAMMADI M.*, SHABANI A., POURKAZEMI M., BARADARAN NOVEIRI S.
 
* GENETICS DEPARTMENT, INTERNATIONAL STURGEON RESEARCH INSTITUTE, RASHT, IRAN
 
Abstract: 

In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identification. We used 8 primer combinations (Eco+3, Mse+4) and a total of 250 bands (size range 40-1000 bp) were generated. Primer combinations of (E-AAT, M-CGAT) and (E-AAG, M-CGAT) produced diagnostic bands in hybrids and parental species. Moreover, the results of genetic identification showed that Bester hybrids are more similar to beluga (Huso huso) (0.68) in comparison with sterlet (Acipenser ruthenus) (0.45).
The results suggested that this technique could be suitable for precise identification of species and inter-generic hybrids like bester.

 
Keyword(s): AFLP, HYBRID IDENTIFICATION, BESTER, BELUGA
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 84
 
Latest on Blog
Enter SID Blog