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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 139 To 139.
 
Paper: 

HYPERTHERMOPHILIC NANOBACTERIA ISOLATED FROM GHEINARCHEH HOT SPRING

 
 
Author(s):  YARIZADEH SHOHREH*, ZARRINI GHOLAMREZA
 
* DEPARTMENT OF BIOLOGY, FACULTY OF NATURAL SCIENCES, UNIVERSITY OF TABRIZ, TABRIZ, IRAN
 
Abstract: 

Background: The aim of this research was to isolate and identify the thermophilic Nano bacteria from Gheinarcheh hot spring.
Methods: Microbial mats were found from Gheinarcheh hot spring in the north west of Iran (Latitude: 38° 16' 1.3012", Longitude: E 47o 48' 35.1123") and we tried to isolated protease producing thermophilic microorganisms. To identify features of these mats, synthetic media was used and incubated in temperature range between 60 to 1200oC also in autoclave. To describe this microbial biofilms, scanning electron microscopy was applied and DNA extraction was done with method of Sheima and PCR was performed with four universal 16S rRNA primers.
Results: Microbial biofilms were discovered to be present in developmental stages of biofilm formation observed in our samples and was increased in high temperature between 60 until 1200oC and also in autoclave quickly. In triplicate SEM samples has been seen as the nano-sized features in the scale of 70 nm as Nano bacteria. DNA extracted was seen with gel electrophoresis and PCR was not performed with the universal 16S rRNA primers.
Conclusion: The present work involves the isolation and characterization of thermostable Nano bacteria. Superficial and development recognitions of these thermophilic Nano bacteria were identified from Gheinarcheh hot spring.

 
Keyword(s): NANO BACTERIA, HOT SPRING, HYPER-THERMOPHILIC
 
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