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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 137 To 137.
 
Paper: 

ISOLATION AND CHARACTERIZATION OF METHANE- OXIDIZING BACTERIA FROM SOIL

 
 
Author(s):  SANEI MAHDIYEH*, ROAYAEI MOHAMMAD, SOUDI MOHAMMAD REZA
 
* DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCES, SHAHID CHAMRAN UNIVERSITY, AHVAZ, IRAN
 
Abstract: 

Background: The main purpose of current study is isolation and characterization of the bacteria which are capable of methane consumptionas the sole source carbon and energy. Methods: Soil samples were collected from the oil contaminated regions. The bacterial suspension was prepared and inoculated into NMS (Nitrate mineral salts) broth medium. Then atmosphere of the flasks were filled up with 50% of methane and 50% of air. Incubation was performed on the shaker for 10 days in 30oC in the dark condition. In addition, the gas mixture was renewed every 2 days. Then liquid culture samples were spread onto NMS agar plates. The plates were incubated for 2 weeks in a gas-tight jar with similar gas composition as mentioned above. Single colonies that formed on the NMS agar plates were transferred onto fresh NMS agar plates and then incubated for another week. The contamination of samples with non-methane oxidizing bacteria was investigated by incubation of control plates in the absence of methane. Identification of bacteria was carried out using biochemical tests and molecular procedure.
Results: In this research, 2 bacterial strains were isolated from the oil contaminated soils. On the basis of morphological, physiological properties and biochemical characteristics such as phenylalanine deaminase, DNase, hydrolysis of esculin, tween 80, starch, gelatin, and 16S rRNA gene sequencing analysis, the organisms were identified to belong to Achromobacter and Sphingomonas genera.
Conclusion: Microbial prospection for oil and gas (MPOG), is a surface exploration technology based on the detection of anomalies in microbial distribution in soil samples. Geomicrobial prospecting for hydrocarbons is an exploration method based on the seepage of light gaseous hydrocarbons from oil/gas reservoirs towards the surface and their utilization by hydrocarbon-oxidizing bacteria.

 
Keyword(s): MICROBIAL PROSPECTION, METHANE OXIDIZING BACTERIA, OIL, GAS
 
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