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Paper Information

Journal:   MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI)   AUGUST 2001 , Volume 15 , Number 2; Page(s) 103 To 108.
 
Paper: 

PREPARATION OF HIGHLY PURIFIED SOLVENT-DETERGENT COAGULATION FACTOR VII AND FACTOR IX CONCENTRATES FROM PROTHROMBIN COMPLEX (PPSB)

 
 
Author(s):  NASIRI S., REZVAN H., MOUSAVI K., ROUSTAEI M.H.
 
* 
 
Abstract: 

In this study, anion-exchange chromatography was used to purify factor VІІ and factor ІX from prothrombin complex (PPSB), which contains coagulation factors ІІ,VІІ, ІX and X. For this purpose, DEAE-Sepharose CL-6B gel, Pharmacia column XK-26, high flow rate and two stepwise gradients with phosphate citrate buffer were used. The yield of the two lyophylized products, factor VІІ and factor ІX concentrates, was 61% and 75%, respectively. Specific activity of factor VІІ increased from 0.16 to 3.06 (purification-fold= 19.1) and specific activity of factor ІX increased from 1.2 to 4.9 (purification-fold= 4.1). Results of electrophoresis on agarose gel-as well as immunoelectrophoresis-indicated higher purity of factor VІІ and factor ІX compared to PPSB. Thrombogenicity of the two products were within the normal range defined for PPSB. In order to improve viral safety, solvent-detergent treatment was performed prior to further purification. Factor VІІ concentrate is used for patients with factor VІІ deficiency and also for hemophilia patients with inhibitors. Factor ІX concentrate is used for treatment of hemophilia B patients.

 
Keyword(s): FACTOR VII, FACTOR VIIA, FACTOR IX, HEMOPHILIA, PPSB, VIRAL INACTIVATION
 
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