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Paper Information

Journal:   IRANIAN POLYMER JOURNAL (ENGLISH)   2002 , Volume 11 , Number 5; Page(s) 333 To 338.
 
Paper: 

EFFECT OF SURFACTANTS ON ENZYMATIC HYDROLYSIS OF CELLULOSIC FABRIC

 
 
Author(s):  HEMMATINEJAD N.*, VAHABZADEH F., KORDESTANI S.S.
 
* Department of Textile Engineering, Amir Kabir University of Technology, Tehran, I.R. Iran
 
Abstract: 
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The effect(s) of four different types of surfactants on the enzymatic hydrolysis of cellulosic fabric was studied. Amount of reducing surgar produced during the reaction was used as a measure of extent of the hydrolysis. The non-ionic surfactant increased the rate of the reaction by approximately 12% while in the presence of amphoteric, anionic and cationic surfactants the rate of the hydrolysis decreased by 36, 38, and 50%, respectively. The physical properties, the tensile strength and the crease recovery (angle) of the cellulosic fabrics were measured. In the presence of non-ionic surfactant, the tensile strength loss for cellulase treated fabric was increased by 17%. The loss of tensile strength did not change using cationic surfactant while in the presence of amphoteric and anionic surfactants, the strength loss decreased rather insignificantly. Thickness measurement of the fabrics was also performed and the results obtained were in agreement with those from tensile strength loss. Results from the weight loss measurement followed the same trend as that of the thickness measurement. All the results obtained from measuring the physical properties of the enzymatic treatment of the cellulosic fabric were in agreement with those from reducing sugar measurements. Moreover, the Michaelis-Menten equation which has been used in the previous work of this group, was also used in the present study and the maximal velocity, V" max", and the half-saturation constant, K" m both, were calculated. In order to measure the catalytic specificity of the cellulase on this hydrolytic reaction on the tested fabrics, the ratio of V" max/K" m", was determined. The catalytic specificity for the fabrics treated with non-ionic surfactant was increased, although this ratio for the fabrics treated with amphoteric, cationic, and anionic decreased. The performance of these surfactants in relation to this enzymatic reaction was discussed.

 
Keyword(s): CELLULASE, ENZYMATIC HYDROLYSIS, REDUCING SUGAR RELEASE, CELLULOSIC FABRIC, SURFACTANT
 
References: 
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