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Paper Information

Journal:   JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES   AUGUST 2013 , Volume 21 , Number 3; Page(s) 98 To 108.
 
Paper: 

INVESTIGATION OF ISCHEMIC STROKE MECHANISM BY ANALYZING HUMAN BRAIN PROTEOME

 
 
Author(s):  OMIDI R., ZALI H.*, REZAEI TAVIRANI M., MODARA F.
 
* FACULTY OF PARAMEDICAL SCIENCES, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Introduction: Stroke is the third cause of death in the world. Ischemic stroke can be observed in the 85 to 90% of strokes. In the condition, cerebral blood flow due to the blockage of blood vessels is interrupted and a very complex process, literally called the cascade of ischemic, begins to occur at the cellular and tissue level, which eventually leads to tissue damage and the expression of ischemic stroke. Approaches in this area are weak and a further knowledge of the molecular mechanisms contributing to more effective diagnosis and treatment. Of the valuable tools for the understanding of molecular processes involved in ischemic stroke is proteomics method that provides us a snapshot of the proteins expressed at the cellular or tissue level, and can give us valuable information for the characterization of its biological processes.
Materials & Methods: In this study, the data from human brain proteome changes in ischemic stroke using two-dimensional gel electrophoresis and mass spectrometry were analyzed by DAVID program. The lists of genes with altered protein expression were obtained from the article “the proteome of human brain after ischemic stroke”. Findings were analyzed by the DAVID bioinformatics software. The association between ischemic stroke genes and other diseases, stroke clustering of proteins based on biological processes and intra cellular sites and molecular functions of the genes was performed via "GO" database.
Findings: Of 39 genes involved in ischemic stroke, totally four biological pathways chart were obtained which were associated with central nervous system diseases such as Parkinson, Alzheimer and Huntington. Most of the proteins were located in mitochondria and more significantly involved in the response to oxidative stress, apoptosis and necrosis processes.
Discussion & Conclusion: The data from two-dimensional gel electrophoresis-based proteomics experiments are important for understanding of the processes that occur during ischemic stroke. Proteins identification may be used as treatment targets or as biological markers for diagnostic and prognostic purposes in the management of stroke.

 
Keyword(s): TWO-DIMENSIONAL ECTROPHORESIS, ISCHEMICSTROKE, DAVID PROGRAM, PROTEOME
 
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