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Paper Information

Journal:   KOWSAR MEDICAL JOURNAL   Spring 2002 , Volume 7 , Number part 1; Page(s) 27 To 34.
 
Paper: 

THE EFFECTS OF THERAPEUTIC ULTRASOUND IN COMBINATION WITH VARIOUS DOSES OF BLEOMYCIN SULFATE ON MICRONUCLEI FORMATION IN GO-HUMAN-LYMPHOCYTES

 
 
Author(s):  MOZDARANI H.*, YOUSEFIAN B., MOKHTARI DIZAJI M.
 
* Faculty of Medicine, Tarbiat Modarress University of Medical Sciences, Tehran, IR IRAN.
 
Abstract: 
The clastogenic and cytogenetic effects of continuous ultrasound alone or in combination with various doses of bleomycin sulfate were evaluated on human GO-Iymphocytes. Lymphocytes were separated and exposed to 0.5 W continuous ultrasound alone or in the presence of various doses of bleomycin sulfate before culture initiation. To arrest cells in cytokinesis, cells were exposed to 5 µg/ml cytochalasin - B then micronuclei were prepared according to standard methods. Slides were stained in 10% Giemsa and 1000 binuclei were scored for the presence of micronuclei for each treatment. Results indicated that the frequency of micronuclei increases following increasing doses of bleomycin and was significantly different from control and sham control groups (P<0.05). Ultrasound alone did not produce high frequency of micronuclei different from sham controls significantly. However, when cells were exposed to ultrasound in the presence of 2-4 mg/ml bleomycin, a prefound effect but not more than additive, was observed. Combinations of ultrasound and 6 mg/ml blemycin led to a synergistic clastogenic effect in lymphocytes (1.7 times more than the total micronuclei observed for each treatment). The mechanism by which ultrasound enhances the effects of bleomycin is not fully known. It might bring changes in the cytoplasmic membrane transportation so that larger numbers of drug molecules are entered into the cell causing more DNA damage and hence micronulei formation.
 
Keyword(s): THERAPEUTIC ULTRASOUND, BLEOMYCIN SULFATE, CYTOCHALASIN - B BLOCKED CELL, MICRONUCLEI, GO - HUMAN LYMPHOCYTES.
 
References: 
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