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Paper Information

Journal:   PHARMACEUTICAL SCIENCES   Spring-Summer 2001 , Volume - , Number 1; Page(s) 1 To 8.
 
Paper: 

STUDYING POSSIBLE MECHANISMS OF ACUTE VASORELAXANT EFFECT OF 17B-ESTRADIOL

 
 
Author(s):  BABAEI HOSSEIN, MOHAJEL NAEBI ALI REZA
 
* 
 
Abstract: 

Cardiovascular diseases are the main cause of mortality in most countries Comparing with age matched men the incidence of these diseases in fettling women and menopause women who take estrogen is low. This suggests a protective effect for estrogen in cardiovascular system. However the exact mechanism of this protective effect is not elucidated and is controversial. One of the possible mechanisms is the direct relaxing effect of es1rogen on vascular smooth muscle. The present study aims to identity the possible mechanism(s) of the vasorelaxant effect of 17B-estradiol on rat aorta.
Rings of aorta 3-5 mm wide were prepared from male Wistar rats (250-350 g) and equilibrated in Krebs' solution under 2g tension (37°C; 95% O2, 5% CO2) for 60 min. Rings were contracted with. PGF2 (10 µM), an approximately EC80 concentration. When contraction was stable 17 B-estradiol was applied for 40 minutes. Relaxation was expressed as % reversal of contraction. For studying the possible effect of endothelium, prostaglandins, nitric oxide and classic estrogen receptors in the vasorelaxant effect of 17B-estradiol, the relaxant effect was assayed again in the absence of endothelium, and in the presence of indomethacin. N-nitro-L-arginine methyl ester (L-NAME). methylene blue, puromycin, actinomycin-D, cyclohexamide and tamoxifen.
Denuding the endothelium of aortic rings or incubating them with indomethacin, L-NAME, methylene blue, puromycin, actinomycin-D, cyclohexamide and tamoxifen did not alter the vasorelaxant effect of 17B-estradiol significantly (P>0.05). Therefore, it would suggest that this acute relaxing effect of estrogeil is independent of endothelium, prostaglandin production, NO synthesis and genomic pathways. Further studies are needed to clarify the mechanism(s) by which 17B-estradiol relaxes vascular smooth muscle.

 
Keyword(s): RAT AORTA, VASORELAXANT EFFECT, 17B-ESTRADIOL
 
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