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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   Fall-Winter 2002 , Volume 19 , Number 63-64; Page(s) 45 To 50.
 
Paper: 

IDENTIFICATION OF HELICOBACTER PYLORI DIRECTLY FROM BIOPSY SPECIEMENS BY UREC (GLMM) PCR WITHOUT CULTURE AND COMPARISON WITH BACTERIOLOGICAL METHODS

 
 
Author(s):  NAVAB AKBAR F., SALEHI A.
 
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Abstract: 
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Helicobacter pylori is a microaerophilic gram negative fastidious bacterium. It has been associated with chronic gastrits, peptic ulcer and gastric carcinoma. In the present study by urec PCR directly without culture 79 biopsies were studied and compared with bacteriological methods including gram stain, rapid urease test and mlture. 64 cases were positive on PCR at sensitivity 91.4% and specifity 100% 73 cases were positive on rapid urease test at sensitivity 100% and specifity 66.7% and 71 cases were positive on gram stain at sensitivity 88.9% and specfity 100% 40 cases were positive on culture at sensitivity 57.1% and specifity 100%.
Based on the results obtained by urec PCR we can indifity H.pylori directly without culture on biposy speciemens. In comparison with bacteriologieal tests urec PCR enjoyes a higher dgree of sensitivity and specifity. In addition to identifying the bacterium, we can use methods such as RFLP with restriction endonuclease on products of PCR to determine genotyping, reinfection infection with new strain, recrudesencece or drug resitance. Also using by this methods we can study in malignant cases embed tissues including MALT and find the role of H.pylori in the disease as retrorespective study.

 
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