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Paper Information

Journal:   MODARES JOURNAL OF MEDICAL SCIENCES (PATHOBIOLOGY)   SPRING 2012 , Volume 15 , Number 1; Page(s) 81 To 91.
 
Paper: 

APPLICATION OF SEMI-NESTED PCR RFLP FOR DETECTION AND GENOTYPING GBV-C AMONG HBV-INFECTED PATIENTS

 
 
Author(s):  YAZDANI LALEH, RAVANSHAD MEHRDAD*, KHANLARI ZAHRA, YAGHOOBI RAMIN
 
* DEPARTMENT OF VIROLOGY, FACULTY OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN, P.O.CODE: 1411713116
 
Abstract: 

Objective: GBV-C is a virus transmissible via blood transfusion and sexual routes. Because of the shared transmission routes in GBV-C and hepatitis B virus (HBV) we have attempted to detect GBV-C in HBsAg-positive patients using the more sensitive semi-nested PCR. We used restriction fragment length polymorphism (RFLP) to determine genotype.
Methods: A total of 100 serum samples were collected from HBsAg-positive patients from 1389-1390. After designing specific primers and optimizing the semi-nested PCR, sequences of PCR products were analyzed by Neb Cutter software. Restriction enzyme sites were determined and suitable enzymes selected for RFLP.
Results: Semi-nested PCR shows acceptable sensitivity for the detection of GBV-C and its genotype determination. This technique is more affordable than other techniques.
Conclusion: There appears to be a high rate of GBV-C infection among Iranian HBV patients. In this study, the majority of patients co-infected with GBV-C were of HBV genotype 2, which is similar to the pattern seen in the US and Europe.

 
Keyword(s): GBV-C, HBS-AG POSITIVE, RFLP, GENOTYPING, SEMI-NESTED PCR
 
References: 
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