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Paper Information

Journal:   AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)   JULY-SEPTEMBER 2012 , Volume 4 , Number 3 (14); Page(s) 131 To 141.
 
Paper: 

3'-RACE AMPLIFICATION OF AMINOPEPTIDASE N GENE FROM ANOPHELES STEPHENSI APPLICABLE IN TRANSMISSION BLOCKING VACCINES

 
 
Author(s):  BOKHARAEI HANIEH, RAZ ABBASALI, ZAKERI SEDIGHEH, DINPARAST DJADID NAVID*
 
* MALARIA AND VECTOR RESEARCH GROUP (MVRG), BIOTECHNOLOGY RESEARCH CENTER (BRC), PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 
Background: Because of the lack of an effective and economical control strategy against malaria (the most devastating infectious disease in developing countries) Transmission-Blocking Vaccines (TBVs) concept has been raised in recent years, promising a more efficient way to malaria control. TBVs aim at interfering and/or blocking pathogen development within the vector, halting transmission to non-infected vertebrate host. Aminopeptidase N (APN) is one of the most potent proteins in parasite development in Anopheles malaria vectors, which is strongly co-localized with human malaria parasites in the mosquito midgut epithelium. Therefore, Aminopeptidase N is one of the best choices for a new TBV.
Methods: In this study for the first time we used 3'-RACE to amplify APN gene in Anopheles stephensi (An.stephensi), a major malaria vector in Iran, Indian subcontinent up to China by using different sets of primers including exon junction, conserved and specific region primers.
Results: Full length of APN was sequenced stepwise, which could be applied in designing a new regional TBV and act as an essential component of malaria elimination program in An. stephensi distribution areas.
Conclusion: Primers design and method modification should be set up exactly in approach based amplifications. From results we came to this conclusion that that 3'-RACE could be applied to amplified key regions which are beyond reach.
 
Keyword(s): 3'-RACE, ANOPHELES STEPHENSI, APN, AMINOPEPTIDASE N, MALARIA, TRANSMISSION BLOCKING VACCINE
 
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