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Paper Information

Journal:   JOURNAL OF IRANIAN ANATOMICAL SCIENCES   WINTER 2012 , Volume 9 , Number 37; Page(s) 270 To 278.
 
Paper: 

EFFECT OF NEUROTROPHIN-3 ON DIFFERENTIATION OF RAT HAIR FOLLICLE STEM CELLS INTO NEURAL LIKE CELLS

 
 
Author(s):  MOGHANI GHOROGHI F.*, NOBAKHT M., ESMAEILZADE B., BAKHTIYARI M., HEJAZIAN L.B.
 
* HISTOLOGY AND NEUROSCIENCE DEPARTMENT, SCHOOL OF MEDICINE, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Purpose: The aim of this study was to evaluate the effect of NT-3 on the decrease of the differentiation time of bulge stem cells of rat hair follicle from neuron like cells.
Materials and Methods: The bulge region of the rat whisker was isolated from and cultured in DMEM/F12 supplemented with epidermal growth factor (EGF) in 3 groups for 7, 8 and 9 days .Then 10 ng/ml NT-3 was added to each group for 3 days. Finally, cell differentiation was assessed by immunocytochemistry using
β-tubulin antibody and the result was compared with control groups.
Results: According to our results hair follicle bulge stem cells expressed CD34 and Nestin in 7-9 and 10-12 first days after cultivation respectively. By using NT-3 duration differentiation process the cells showed positive reaction to
β-tubulin antibody.
Conclusion: The results indicate that NT-3 can affect on differentiation speed even in less than 10 days.

 
Keyword(s): BULGE OF HAIR FOLLICLE, CD34, NESTIN, NT-3, ΒⅢ-TUBULIN
 
References: 
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