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Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCE (UNIVERSITY OF TABRIZ)   2009 , Volume 19 , Number 2; Page(s) 253 To 261.
 
Paper: 

EFFECT OF COLCHICINE ON NUCLEI DIAMETER, YIELD AND SOME MORPHOLOGICAL CHARACTERISTICS OF OYSTER MUSHROOM (PLEUROTUSE OSTREATUS)

 
 
Author(s):  HOSSEINI S.Z., KARIMZADEH G.*, MOHAMMADI GOLTAPEH E.
 
* PLANT BREEDING AND BIOTECHNOLOGY DEPARTMENT, FACULTY OF AGRICULTURE, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Oyster Mushroom (Pleurotuse ostreatus) is one of the industrial, pharmaceutical and commercial edible basidiomycetes. In this study, we examined colchicine treatment for the production of autopolyploid P. ostreatus. For production of mycelium, the spores were cultured on MEA culture medium. For colchicine treatment, the mycelia were then cultured in Czapek liquid culture medium containing glucose (0.1% w v-1) and peptone (0.5% w v-1) adding 0, 0.05, 0.1 and 0.3% of colchicine solutions to the medium. The mycelia were stained with Safranin-O and their nuclei diameters were measured, using IX170 Olympus invert light microscope interfaced with a DP50 digital camera. The nuclei were increased in diameter at different degrees in different concentration of colchicine solutions. Such increment response indicates the increase of nuclei DNA content, verifying the autopolyploid formation of P. ostreatus. From the produced mycelia spawn were made and in order to evaluate some important morphological characteristics, they were used for inoculating wheat straw. After the start of fruiting stage, yield, diameter of basidiocarp and length of stem were measured. The yield was increased up to %82 compared to the control, the stem and the diameter of basidiocarp also enlarged in the colchicines-treated P. ostreatus.

 
Keyword(s): COLCHICINE, NUCLEI DIAMETER, OYSTER MUSHROOM, POLYPLOIDY
 
References: 
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