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Paper Information

Journal:   IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)   JUNE 2011 , Volume 36 , Number 2; Page(s) 122 To 127.
 
Paper: 

FUNCTIONAL AND DEVELOPMENTAL ANALYSIS OF CD4+CD25+ REGULATORY T CELLS UNDER THE INFLUENCE OF STREPTOCOCCAL M PROTEIN IN RHEUMATIC HEART DISEASE

 
 
Author(s):  ABDUL AUHAIMEN N.*, AL KAABI Z.I.
 
* DEPARTMENT OF MICROBIOLOGY, ALNAHRAIN COLLEGE OF MEDICINE, BAGHDAD, IRAQ
 
Abstract: 

The purpose of this study was to determine the role of streptococcal M protein in naturally-occurring CD4+CD25+ regulatory T cells (nTregs) function and development in rheumatic heart disease in Iraqi patients. Streptococcus pyogenes was isolated for subsequent M protein extraction. Also, peripheral blood nTregs and CD4+ T cells were isolated by using Magnetic Cell Separation System. Tissue culture for isolated cells was performed in the presence and absence of M protein. Cell count was performed, and tumor necrosis factor alpha (TNF-a) and interleukin-4 (IL-4) were determined in culture supernatant using ELISA system. There was a significant positive correlation (P<0.01) between the number of proliferated nTregs and CD4+ T cells in the presence as well as the absence of streptococcal M protein. Moreover, there was a significant negative correlation between the mean number of nTregs and CD4+ T cells in mixed culture system in the absence of M protein (r=- 0.995). There was also a positive, but not significant (P>0.05), association (r=0.353) between the mean number of nTregs and CD4+ T cells in the presence of M protein. The M protein stimulated CD4+ T cells to produce IL-4 in very little amount (<4 pg/ml) in all samples. Compared to the production of IL4, TNF-a was produced in higher concentrations in the culture supernatants. The findings of the study indicate that streptococcal M protein has an important role in increasing the proliferation of CD4+CD25+regulatory T cellsand CD4+ T cells. However, CD4+CD25+ regulatory T cells have lower suppressive activity against CD4+ T cells in the presence of M protein.

 
Keyword(s): RHEUMATIC HEART DISEASE , CD4+CD25+ REGULATORY T CELLS , M PROTEIN
 
References: 
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