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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   WINTER 2009 , Volume 3 , Number 4; Page(s) 57 To 63.
 
Paper: 

NO EXISTENCE OF ALLELIC POLYMORPHISM IN BOOROLA (FECB) AND INVERDEL (FECXI) GENES IN LURI-BAKHTIARI SHEEP BREED

 
 
Author(s):  AMIRI E., RAHIMI G., VATANKHAH M.*
 
* RESEARCH CENTER OF AGRICULTURE AND NATURAL RESOURCES, CHARMAHAL, BAKHTIARI PROVINCE
 
Abstract: 

High rate of ovulation and litter size are the most important affecting factors in reproduction system and subsequently increases economical efficiency in sheep breeding industry. This study was carried out for detection of mutation in FecB and FecXI genes in Lori-Bakhtiari sheep breed. It has been reported that these major genes can increase ovulation and also twining rate in different sheep breed. Blood samples were collected from 165 individual for identification of polymorphisms at these loci. DNA was extracted using modified salting out method. Polymorphism chain reaction (PCR) was used to amplify desired fragments at these loci using two pairs of specific primers. Each of specified primer pairs amplified a fragment with 190 and 154 bp at FecB and FecXI loci, respectively. For genotyping of the samples, the PCR products were electrophoresed on 3% agarose gel after digestion with endonuclease AvaII for FecB and XbaI for FecXI. In the case of mutation, two fragments of 30, 160 and 30, 124 bp could be detected in FecB and FecXI loci, respectively. The genotyped samples at the present study did not confirm the incidence of mutant allele (-), since all samples showed monomorph (+/+) genotypes. Regarding the phonotypic record in this breed, the obtained results indicates that the genetic factor responsible for twining or multiple lambing rate is not related to reported mutated alleles at Booroola or Inverdel major genes and we should search for other genes in this breed.

 
Keyword(s): SHEEP, LORI-BAKHTIARI, FECB, FECXI, GENE, PCR
 
References: 
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