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Paper Information

Journal:   IRANIAN JOURNAL OF BIOTECHNOLOGY   JANUARY 2011 , Volume 9 , Number 1; Page(s) 56 To 62.
 
Paper: 

GENETIC POPULATION STRUCTURE OF HAWKSBILL TURTLE (ERETMOCHELYS IMBRICTA) USING MICROSATELLITE ANALYSIS

 
 
Author(s):  ZOLGHARNEIN H., SALARI ALIABADI M.A.*, FOROUGMAND A.M., ROSHANI S.
 
* DEPARTMENT OF MARINE BIOLOGY, FACULTY OF MARINE SCIENCE, KHORRAMSHAHR UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY, KHORRAMSHAHR, KHUZESTAN, I.R. IRAN
 
Abstract: 

Information on the genetic structure of marine species is essential for stock improvement programs. In order to analyses the genetic diversity of the Hawksbill turtle (Eretmochelys imbricte) by the microsatellite genetic method, 64 samples were caught from the beaches located in Kish and Qeshm islands. Polymerase chain reactions (PCR) of genomic DNA extracted from the samples were carried out using 5 pairs of microsatellite primers. The results of this study indicated that all 5 pairs of primers were polymorphic. Average numbers of real allele and effective allele were 4.90 and 2.99, respectively. Average rate of observed heterozygosity was 0.570 and that for expected heterozygosity was 0.616. Study of the Hardy-Weinberg equilibrium was shown the entire locus had not equilibrium except Cm3 and Ei8 locus in Kish area. Fst (0.166) and Rst (0.634) calculated by the Analysis of Molecular Variance (AMOVA) test illustrated that there are separate populations of Hawksbill turtle in this part of the Persian Gulf (Kish and Qeshm islands). It seems that Kish’s turtles live under better conditions in contrast to their Qeshm counterparts. Diminution of genetic variation within examined population decreases its adaptation to environmental alterations. We identified two different E. imbricte populations from north of the Persian Gulf.

 
Keyword(s): MICROSATELLITES, GENETIC VARIATION, POLYMORPHISM, ERETMOCHELYS IMBRICTE, PERSIAN GULF
 
References: 
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