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Paper Information

Journal:   RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)   APRIL 2008 , Volume 3 , Number 1; Page(s) 59 To 63.
 
Paper: 

EFFECTS OF LIGHT AND DIFFERENTIATION ON GINGEROL AND ZINGIBERENE PRODUCTION IN CALLUS CULTURE OF ZINGIBER OFFICINALE ROSC.

 
 
Author(s):  ANASORI P., ASGHARI G.*
 
* DEPARTMENT OF PHARMACOGNOSY, SCHOOL OF PHARMACY AND PHARMACEUTICAL SCIENCES, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, I.R.IRAN
 
Abstract: 

Ginger (Zingiber officinale Rosc.) is a herbaceous, rhizomatous, perennial plant, which its rhizomes are used as an important spices and medicine all over the world. The aromatic principles include zingiberene, while the pungent principles are known as gingeroles. Callus culture of the plants can produce the same compounds exist on their parent plants. The aim of this work was to evaluate the effect of light and differentiation on gingerol and zingiberene production in callus cultures of Z. officinale. A sterile in vitro plant was prepared by sterilization and subculture of buds, excised aseptically and inoculated into sterile culture jars containing Murashige and Skoog’s (MS) medium and were incubated at 25 ± 2°C under a 16/8 h light/dark cycle. Then different parts of the sterile plant were inoculated in MS medium supplemented with different concentrations of 2,4-dichlorophenoxy acetic acid and kinetin. The jars were separated into two groups, one incubated in dark and the other one in light environment, permanently. Production of metabolites was evaluated by TLC. Some of the metabolites were produced only in presence of light. No gingerol and zingiberene was detected on TLC plates of the dedifferentiated callus grown in light or dark environment. It seems that the production of gingerol and zingiberene in callus culture of Z. officinale is correlated with some sort of differentiation.

 
Keyword(s): ZINGIBER OFFICINALE, CALLUS, LIGHT, DIFFERENTIATION
 
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