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Paper Information

Journal:   IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY   SPRING 2009 , Volume 3 , Number 1; Page(s) 25 To 30.
 
Paper: 

THE ROLE OF RDXA MUTATION IN METRONIDAZOLE RESISTANCE IN HELICOBACTER PYLORI STRAINS

 
 
Author(s):  KARGAR MOHAMMAD*, BAGHERNEZHAD MARYAM, DOUSTI ABAS
 
* ISLAMIC AZAD UNIVERSITY, DEPARTMENT OF MICROBIOLOGY
 
Abstract: 

Background and objectives: Metronidazole is a key component for treatment of peptic ulcer caused by Helicobacter pylori infection. The aim of this study was to determine the role of rdxA gene in metronidazole resistance in H.pylori strains isolated from Hajar hospital of Shahrekord, Iran.
Materials and methods: This cross-sectional study was done on 263 patient who referred to endoscopy department of Hajar hospital of Shahrekord on summer 2007. H.pylori was identified using gram stain, urease, catalase, oxidase tests and PCR method. Metronidazole Resistance was evaluated according to Standard Clinical and Laboratory Standards Institute (CLSI). Specific primers were used for detection of 200bp deletion in sensitive, semi-sensitive and resistance strains.
Results: Out of 84 isolated strains, 49 (58.33%) and 7 (%8.33) were resistant and semi-sensitive to metronidazole respectively. However 200bp deletion in rdxA gene was observed in only 2 strains. Statistical analysis indicated that there was no relation between metronidazole resistance, clinical criteria and rdxA gene deletion.
Conclusion: Regarding to low frequency of rdxA gene mutation observed in our survey, the study of other possible molecular mechanisms involved in resistance to metronidazole is recommended.

 
Keyword(s): HELICOBACTER PYLORI, METRONIDAZOLE RESISTANCE, RDXA GENE
 
References: 
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