Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF BIOLOGY   SUMMER 2009 , Volume 22 , Number 2; Page(s) 282 To 292.
 
Paper: 

INVESTIGATION ON INHERITANCE OF GLYPHOSATE TOLERANCE IN T1 GENERATION OF TRANSGENIC RAPESEED (BRASSICA NAPUS L.) WITH THE MUTATED BACTERIAL EPSPS GENE

 
 
Author(s):  MOHAMMADALI TAJRISHI M., SALMANIAN ALI HATEF*, MOUSAVI AMIR, KAHRIZI D.
 
* NATIONAL INSTITUTE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY, TEHRAN, I.R. OF IRAN
 
Abstract: 

In transgenic plants, the faithful inheritance and expression of transgenes are particularly important properties. In this study analyses were performed on seeds that produced by T0 glyphosate-tolerant rapeseed plants. The T0 generation plants contains a mutated EPSPS gene that has a highly linked with nptII gene. These seed screened on MS medium containing 15 mgL-1 kanamycin and explants with green leaves and expanded roots considered as tolerant plants. Results showed that mutated EPSPS gene expresses in T1 canola plants decrease the harmful consequences of glyphosate. The statistical c2 analysis (p-value 0.05) revealed that the segregation of the transgenes to T1 progeny suggested a Mendelian pattern as single dominant locus. In molecular analysis, the presence and transcription of EPSPS genes studied by PCR and RT-PCR, respectively. The T1 plants were fully fertile, with no apparent phenotypic abnormalities.

 
Keyword(s): RAPESEED, GLYPHOSATE, MUTATED EPSPS, TRANSGENE STABILITY
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 74
 
Latest on Blog
Enter SID Blog