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Paper Information

Journal:   SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL   FALL 2007 , Volume 3 , Number 3 (16); Page(s) 91 To 99.
 
Paper: 

EVALUATION OF GEL DIFFUSION TEST FOR DIAGNOSIS OF UNILOCULLAR HYDATID CYST IN EXPERIMENTALLY INFECTED SHEEP

 
 
Author(s):  RAZI JALALI MOHAMMAD HOSSEIN, GHORBANPOUR NAJAFABADI M., HOGHOUGHIRAD N.
 
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Abstract: 

Hydatid cyst is one of the zoonotic diseases which has losses on animal and human populations. One of the most important problems in hydatidosis is the diagnosis of the disease in human and animals. Various assays have been developed and used for the detection of specific antibodies in human and animals sera with variable results. Since the evaluation of serological tests in human has restriction, this study was carried out in experimentally sheep hydatidosis. In this study, periods of antibodies appearance were surveyed by Gel Diffusion test which has high specificity in hydatid cyst diagnosis. The hydatid fluid (HF) and protoscolces were aseptically obtained in laboratory. Each dog was given 15,000 viable protoscoleces. About two months later, as soon as the taenid eggs were observed in the dogs stools, the dogs were autopsied after euthanasia. The small intestines were opened and Echinococcus granulosus were collected and placed in physiologic saline. The gravid proglottids were crushed and the eggs were released and counted. About 2000 eggs were orally administred to each of 13 sheep. Six other sheep were kept as controls and physiological saline were fed to them. All sheep were bleeded each week. And all sera were kept in -200c until use. All sera samples were then examined with Gel Diffusion test. The sensitivity of Gel Diffusion test, in detecting antibodies in the sera of infected sheep was nil during five weeks of post- infection (P.i). The sensitivity increased to 15% in the sixth week and reached its maximum (69%) only in the 14th week of p.i. then it decreased in the following weeks. However, the specificity of the test was 100% by examining the noninfected sheep sera through the experiment. It seems that the capability of diagnosis of antiydatid antibody depends on cyst growth stage. Although positive results are very valuable, the negative results are not indicative of the absence hydatid cyst in sheep, and doing other serologic tests is necessary with high sensitivity for hydatid cyst diagnosis.

 
Keyword(s): SHEEP HYDATIDOSIS, ECHINOCOCCUS GRANULOSUS, GEL DIFFUSION
 
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