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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   SUMMER 2009 , Volume 11 , Number 2 (42); Page(s) 220 To 227.
 
Paper: 

OPTIMIZED METHOD FOR BOVINE BLASTOCYST VITRIF ICATION USING A SIMPLE HAND-MADE CRYOTIP

 
 
Author(s):  ASGARI VAJIHEH, FOROUZANFAR MOHSEN, HOSSEINI SEYED MORTEZA, HAJIAN MAHDI, MOULAVI FARIBA, ABEDI PARVANEH, HOSSEINI LALEH, SADEGHI HOSSEIN, BAHRAMIAN H., NASRESFAHANI MOHAMMAD HOSSEIN*
 
* REPRODUCTION AND DEVELOPMENT DEPARTMENT, ROYAN INSTITUTE FOR ANIMAL BIOTECHNOLOGY, ACECR, ISFAHAN, IRAN
 
Abstract: 

Objective: This study introduced a simple method for bovine blastocyst vitrification.
Materials and Methods: Bovine blastocysts were produced in vitro by means of a whole co-culture system with vero cells. The blastocysts were randomly divided 1:3 into either vitrification (100 blastocysts) or control (43 blastocysts) groups. For vitrification,expanded – blastocysts were incubated first in equilibration medium for 8 minutes and then in the vitrification solution for 1 minute. The blastocysts were then loaded in the tip of a handmade cryotip for immediate - deep freezing in liquid nitrogen. Frozen embryos were then warmed by directly immersing the tips in sequential warming solutions. Warmed embryos were cultured for a further period of 48 hours when the ratios of re-expansion, hatching and degeneration were compared with the control group.
Results: After warming, in the vitrified and control groups the ratios of re-expansion were 78.5% ± 0.067 and 81.6% ± 0.072, the ratios of hatching were 43.7% ± 0.083 and 49.8% ± 0.089 and the ratios of degeneration were 36% ± 0.082 and 22.3% ± 0.087, respectively, which were not significantly different between the two groups.
Conclusion: Post - warming survival of the vitrified and non - vitrified embryos were not significantly different, handmade cryotips can be used as an efficient and feasible device for bovine blastocysts vitrification.

 
Keyword(s): BLASTOCYST, WARMING, SURVIVAL
 
References: 
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