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Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   FALL 2007 , Volume 62 , Number 4; Page(s) 215 To 221.
 
Paper: 

CLONING OF FUSION (F) PROTEIN GENE OF PESTE DES PETITS RUMINANTS VIRUS (PPRV) IN SECRETORY PICHIA PASTORIS VECTOR

 
 
Author(s):  LOTFI M.*, KEYVANFAR H., SEYFIABAD SHAPOURI M.R., GOUDARZI HOSSEIN, POURBAKHSH S.A., KAMALZADEH M.
 
* DEPARTMENT OF QUALITY CONTROL RAZI VACCINE AND SERUM RESEARCH INSTITUTE, KARAJ, IRAN
 
Abstract: 
With advent and development of DNA recombinant technology and advantages of p. pasloris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cloned into pPICZaA a secretory expression vector of P. pasloris for first time. The insertion was proved by both production of a 218 bp segment in Nested PCR and isolation of gene from construct by restriction enzyme (XbaI). Finally, it was sequenced. In conclusion, after the expression of fusion (F) gene in p. pastoris expression system, it can be used in production of recombinant vaccine against PPR disease.
 
Keyword(s): PESTE DES PETITS RUMINANTS VIRUS (PPRV), FUSION PROTEIN, CLONING, P.PASLORIS, YEAST EXPRESSION VECTOR
 
References: 
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