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Paper Information

Journal:   IRANIAN JOURNAL OF BIOTECHNOLOGY   OCTOBER 2008 , Volume 6 , Number 4; Page(s) 207 To 213.
 
Paper: 

OPTIMIZATION OF TRANSIENT EXPRESSION OF UIDA GENE IN ANDROGENIC EMBRYOS OF WHEAT (TRITICUM AESTIVUM L. CV. FALAT) VIA PARTICLE BOMBARDMENT

 
 
Author(s):  GHARANJIK SH., MOEINI A.*, MOUSAVI AMIR, ALIZADEH HOUSHANG
 
* DEPARTMENT OF PLANT BREEDING, FACULTY OF AGRICULTURE, TARBIAT MODARES UNIVERSITY, TEHRAN, I.R. IRAN
 
Abstract: 
Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0.6 mm, 1 mm and 1.6 mm), DNA and microcarrier concentrations (0.5 mg of DNA with 150 mg of gold particles or 1.0 mg of DNA with 300 mg of gold particles/bombardment) and bombardment numbers (1x (single) and 2x (double)) were assessed. The effect of high osmoticum in the bombardment medium (0.3 M mannitol and 0.4 M maltose) and the age of embryos were also evaluated. Optimal expression in MDEs was obtained using the following conditions of double bombardment at 1350 psi, 9 cm target distance, a 1 mm gold particle size, 1.0 mg of DNA with 300 mg of gold particles/bombardment, and osmotic pretreatment of 4-6 weeks old embryos using 0.4 M maltose for 6 h before and 16 h after bombardment. The optimized transformation protocol presented in this study is expected to improve development of commercial transgenic wheat lines expressing desirable agronomic traits.
 
Keyword(s): HEXAPLOID WHEAT, TRANSIENT GENE EXPRESSION, GUS, PARTICLE BOMBARDMENT, ANDROGENIC EMBRYOS
 
References: 
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  pdf-File tarjomyar Persian Abstract Yearly Visit 115
 
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