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Paper Information

Journal:   IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)   2001 , Volume 2 , Number 2; Page(s) 220 To 208.
 
Paper: 

THE EFFECTS OF BOVINE VIRAL DIARRHOEA VIRUS ON SPERM PENETRATION AND FERTILIZATION RATES OF IN VITRO MATURED BOVINE OOCYTES

 
 
Author(s):  KAFI FELAVER JANI S.M., MCGOWAN M.R.
 
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Abstract: 

The introduction of bovine viral diarrhoea virus (BVDV) is a special concern for in vitro production of cattle embryos. Two experiments were designed to evaluate the fertilizability of bovine oocytes exposed to BVDV either at the time of oocyte maturation (Exp.I) or at the time of oocyte insemination (Exp.II). The virus inoculums used was derived nom a persistently infected cow. In expo I, follicular oocytes (n=312) recovered from slaughterhouse derived ovaries were divided into either a control group (n=151) which did not become exposed to BVDV and a treatment group (n=161) which was exposed to BVDV (4 to 4.4 log10 TCID50/50ml) at the time of commencement of in vitro maturation. Results of exp.I showed no significant differences between the control and BVDV exposed oocytes in either the cumulus cell expansion rate (79vs 74%; p>0.05) or fertilisation rate (73.7vs 74.5%; P>0.05), respectively. In expo11, in vitro matured oocytes (607) were inseminated either in the absence (control; n=301) or in the presence of BVDV (4 to 4.6 log10 TCID50/50ml n=306). A significant (P<0.05) reduction in the number of fertilized oocytes with two well formed male and female pronuclei was observed in the treatment group compared to the control group (58vs 73%; P<0.05), respectively .This study demonstrates that bovine viral diarrhoea virus has a significant detrimental effect on the formation of male pronuclei during in vitro fertilization of bovine oocytes.

 
Keyword(s): OOCYTE, BOVINE VIRAL DIARRHOEA VIRUS, IN VITRO FERTILIZATION, SPERM PENETRATION
 
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