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Paper Information

Journal:   IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH   2001 , Volume - , Number 6; Page(s) 1 To 23.
 
Paper: 

KARYOTYPIC ANALYSIS OF SEVERAL ANNUAL MEDIC SPECIES (MEDICAGO SPP) USING MULTIVARIATE METHODS OF ANALYSIS

 
 
Author(s):  SHARIAT A., MIRZAEI NADOUSHAN HOSSEIN, GHAMARI ZARE A., SANGTARASH M.H.
 
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Abstract: 

Karyotypic studies were carried out on eleven populations in seven species of annual medics. The length of chromosome arms was measured in at least five well prepared cells at metaphase stage of cell division. The difference between the populations and chromosomes were tested using factorial experiment, based on a completely randomized design. Revealing significant differences between the populations and their chromosome dimensions, the populations relationships were investigated using several multivariate methods of analysis of variance such as cluster analysis and principal components analysis.
Two groups of genotypes having 14 and 16 chromosomes were observed. Cluster analysis based on the short arms length of the chromosomes in the 14 chromosome group assigned three populations of M. polymorpha to one cluster, implying the usefulness of this character and the analysis method in identifying the relationship among the species. In the 16 chromosomes group, M. truncatula and M. radiata showed the most genetic distance based on the total length of the chromosomes.
In principal components analysis, the characteristic of total length of the chromosomes, long arm length and long arm to short arm ratio showed the most influence in the first component formation. The first component also contained more than 98% of the data variation. Long arm to short arm ratio and short arm length characteristics had the most influence on the second principal component formation.

 
Keyword(s): ANNUAL MEDICS, CLUSTER ANALYSIS, PRINCIPAL COMPONENTS ANALYSIS, GENETIC DISTANCE
 
References: 
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