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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   2008 , Volume 10 , Number SUPPLEMENT 1; Page(s) 68 To 69.
 
Paper: 

EFFECTS OF DIFFERENT TIME OF EXPOSURE AND MEDIA WITH VARYING OSMOLARITIES ON VIABILITY AND MOTILITY OF NAJDI BULL SPERM UNDER 5 DEGREES CENTIGRADE

 
 
Author(s):  AFROUGH M.*, BARATI F., PA PAHN A.A.
 
* FAC. VET. MED., CLINICAL SCIENCES, AHVAZ, IRAN
 
Abstract: 

Objective: Storage of semen in refregartor is one of possible method to preserve it. Also for cryopreservation of sperm, it should pass an equilibration time in 5 derees of centigrade with basic cryopreservation medium (without cryoprotectant). Aim of this study is to find the effects if time exposure (15 and 60 minute) and different osmolarities of media (0, 200, 300 and 400 mosm) have any detrimantatl of benificial effect on motility or viability of Najdi bull sperm.
Materials and Methods: Semen collected from 4 mature Najdi bull in station for support of Najdi cow, Khouzistan, Iran. Primary media were made on the base of tryodes with osmolaities of 100, 300 and 500 mosm which were modified to 200, 300 and 400 mosm final osmolarities in combination of semen mixed. Semen was cetrifuged in rich part of centrifuged semen was mixed in aequall proportion with different media. 15 and 60 miute after production of mixute we analysed motility and vitality of sperm by conventional methods.
Results: Results have showed that time of exposure has not any compareble effect on vitality and motility of bull sperm. After 15 minute motility of was significantly reduced in 200(12.5) vs 300 (28.62) (p<0.05) and there was not detrimental effect (p.0.05) on motility at 400 osmolaity medium (18.75) compare to 300 osmol. After 60 minute motility was significantly reduced in 200 (2.82) vs 300 (34.25) (p<0.05) and there was not detrimental effect (p.0.05) on motility at 400 osmolaity medium (27.5) compare to 300 osmol. After 15 minute vitalty of was significantly reduced in 200 (31.25) vs 300 (66.66) (p<0.05) and there was not detrimental effect (p>0.05) on viability at 400 osmolaity medium (45) compare to 300 osmol. After 60 minute vitalty of was significantly reduced in 200 (2.82) vs 300 (34.25) (p<0.05) and there was not detrimental effect (p>0.05) on viability at 400 osmolaity medium (27.5) compare to 300 osmol.
Conclusion: Low temerature is good model for preserving the bull spermatozoa by lowering metabolic requirement and motility of sperm. In cryopreservation methods equlibration time can be reduced with no detrimental effect on sperm motility and viablity compare to long tome.

 
Keyword(s): SPERM, BULL, CRYOPRESERVATION, OSMOLARITY
 
References: 
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